LOCUS       sequence-77807-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pHAGE NFkB-TA-LUC-UBC-GFP-W .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Wilson AA, Kwok LW, Porter EL, Payne JG, McElroy GS, Ohle SJ, 
            Greenhill SR, Blahna MT, Yamamoto K, Jean JC, Mizgerd JP, Kotton DN
  TITLE     Lentiviral delivery of RNAi for in vivo lineage-specific modulation 
            of gene expression in mouse lung macrophages.
  JOURNAL   Mol Ther. 2013 Apr;21(4):825-33. doi: 10.1038/mt.2013.19. Epub 2013 
            Feb 12.
  PUBMED    23403494
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2013.19"; journalName: "Mol Ther"; date: "2013-04"; 
            volume: "21"; issue: "4"; pages: "825-33"
COMMENT     Sequence Label: pHAGE NFkB-TA-LUC-UBC-GFP-W
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..103
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      166..175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(231..262)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    357..474
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(767..811)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agtactcagc gtaagtgatg tccacctcga tatgtgcatc tgtaaaagca attgttccag
       61 gaaccagggc gtatctcttc atagccttat gcagttgctc tccagcggtt ccatcttcca
      121 gcggatagaa tggcgccggg cctttcttta tgtttttggc gtcttccatg gtggctttac
      181 caacagtacc ggaatgccaa gctttttacc aacagtaccg gaatgccaag ctggaattcg
      241 agcttccatt atataccctc tagagtctag atctggaaat tcccggaaat tcccggaaat
      301 tcccggaaat tcccactagt ccaaactgga tctctgctgt ccctgtaata aacccgaaaa
      361 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
      421 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
      481 atgaatactg ccatttgtga attcggcgat accgtcgaga tccgttcact aatcgaatgg
      541 atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc
      601 tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca
      661 ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga
      721 ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt
      781 tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta
      841 aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt
      901 gattccaaat ctgttcca
//
