LOCUS       sequence-77809-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 8, LacZ, final entry vector for
            the assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_8'_XcmI-LacZ
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     113..130
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    247..268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(283..288,289..306,307..313)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    321..337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     326..348
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     345..361
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     345..361
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             357..740
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDIEFPR
                     PPWRPGACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
                     RTDRPSQQLRSLNGEWTRPVAAH"
     promoter        379..397
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      491..500
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      495..504
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(534..553)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(535..553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(560..577)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(560..576)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(569..591)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(778..796)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 atcaagggtc cccaaactca ccctgaagtt ctcaggatcg gtcgagatcc ttacttagtt
       61 acccgggagg atgagggtct cacttaggta ccagcggtgg cgtggcgagc gcagcgagtc
      121 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      181 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      241 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      301 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      361 ccatgattac gccaagctat ttaggtgaca ctatagaata ctcaagctat gcatccaacg
      421 cgttgggagc tctcccatat ggtcgacctg caggcggccg cgaattcact agtgatatcg
      481 aattcccgcg gccgccatgg cggccgggag catgcgacgt cgggcccaat tcgccctata
      541 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      601 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      661 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc
      721 gccctgtagc ggcgcattaa gcgcgccacg ccaccgctgg atccgccggc tgagacctct
      781 atagtgtcac ctaaatcaag cttgctccga cgtccccagg cagaatggcg gttccctaaa
      841 cgagcattgc ttatatagac ctcccattag gcacgcctac cgcccattta cgtcaatgga
      901 acgcccattt gcgtcattgc ccctc
//
