LOCUS       sequence-7781-ex        9525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Bateman JR, Lee AM, Wu CT
  TITLE     Site-specific transformation of Drosophila via phiC31 
            integrase-mediated cassette exchange.
  JOURNAL   Genetics. 2006 Jun . 173(2):769-77.
  PUBMED    16547094
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2006 Jun . 173(2):769-77."
COMMENT     Sequence Label: pUASTP2
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..586)
                     /label=P element 5' end
     protein_bind    776..875
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             complement(join(1590..2204,2275..2406,2627..2942,3004..3658,
                     3733..4006,4401..4472))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4932..4950
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     polyA_signal    4977..5111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5001..5020)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5055..5074
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5536..5556)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5686..5751
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    5942..6041
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     promoter        complement(6137..6375)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(6169..6190)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    6394..6488
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_feature    join(6533..6734,6735..6765)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(7302..7321)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7421..7443
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7481..7499)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7567..7671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7672..7740,7741..8532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7890..7909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8703..9291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9192..9211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca gtactgacgg
      721 acacaccgaa gccccggcgg caaccctcag cggatgcccc ggggcttcac gttttcccag
      781 gtcagaagcg gttttcggga gtagtgcccc aactggggta acctttgagt tctctcagtt
      841 gggggcgtag ggtcgccgac atgacacaag gggttgtgac cggggtggac acgtacgcgg
      901 gtgcttacga ccgtcagtcg cgcgagcgcg actgcatcta cacaaggaac aaacactgga
      961 tgtcactttc agttcaaatt gtaacgctaa tcactccgaa caggtcacaa aaaattacct
     1021 taaaaagtca taatattaaa ttagaataaa tatagctgtg agggaaatat atacaaatat
     1081 attggagcaa ataaattgta catacaaata tttattacta atttctattg agacgaaatg
     1141 aaccactcgg aaccatttga gcgaaccgaa tcgcgcggaa ctaacgacag tcgctccaag
     1201 gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg ggagacagcg aaagagcaac
     1261 tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc gcgcgatttg aaaagtatgt
     1321 atataaaaaa tatatcccgg tgttttatgt agcgataaac gagtttttga tgtaaggtat
     1381 gcaggtgtgt aagtcttttg gttagaagac aaatccaaag tctacttgtg gggatgttcg
     1441 aaggggaaat acttgtattc tataggtcat atcttgtttt tattggcaca aatataatta
     1501 cattagcttt ttgagggggc aataaacagt aaacacgatg gtaataatgg taaaaaaaaa
     1561 aacaagcagt tatttcggat atatgtcggc tactccttgc gtcgggcccg aagtcttaga
     1621 gccagatatg cgagcacccg gaagctcacg atgagaatgg ccagacccac gtagtccagc
     1681 ggcagatcgg cggcggagaa gttaagcgtc tccaggatga ccttgcccga actggggcac
     1741 gtggtgttcg acgatgtgca gctaatttcg cccggctcca cgtccgccca ttggttaatc
     1801 agcagaccct cgttggcgta acggaaccat gagaggtacg acaaccattt gaggtatact
     1861 ggcaccgagc ccgagttcaa gaagaagccg ccaaagagca ggaatggtat gataaccggc
     1921 ggacccacag acagcgccat cgaggtcgag gagctggcgc aggatattag atatccgaag
     1981 gacgttgaca cattggccac cagagtgacc agcgccaggc agttgaagaa gtgcagcact
     2041 ccggcccgca gtccgatcat cggataggca atcgccgtga agaccagtgg cactgtgaga
     2101 aaaagcggta attcggcaat cgttttgccc agaaagtatg tgtcacagcg ataaagtcga
     2161 cttcgggcct ccctcataaa aactggcagc tctgaggtga acacctaaat cgaatcgatt
     2221 cattagaaag ttagtaaatt attaatatgc aaatgtattc taaacaagac ttacatttat
     2281 cgtggcaaag acgttttgaa aggtcatgtt ggtcaggaag aggaagatgg ctccgttgat
     2341 attcatcacg cccacttgcg tgagttgttg gcccaaaaag atgaggccaa tcaagatggc
     2401 aaccatctgc aaattaaaat gttactcgca tctcattaat attcatatct tcaacatgtt
     2461 cgcgagttaa atgaaattta tttattttct gcaaaactat aaactataca tctcattgaa
     2521 aaaaactaag aagggtgtgg aatcaggcaa ttctaactaa aatctagcga atttgtttcc
     2581 aagaattgta agcgttatat catttgtttc cactggaacc actcaccgtt gtctgaataa
     2641 gtcgcacttt tacgaggagt ggttccttga gcaccgacag ccaggatcgc cacaggaccg
     2701 cccggaactg catgaaccag gtggccttgt aggtgtaccc attctccggc tgctccagtg
     2761 gcttctccaa atttttggtg gccaacaact gctccatatc ccgggctact ttgctaatag
     2821 caaaattgtc gcatatcttg gcgatccgat cacgggactc gatctcccgt ccgggcacaa
     2881 cggccaacac ctgtacgtaa aagtccgccg gattgtagtt ggtaggacac tgggcaccca
     2941 cgctggatag gagttgagat gtaatgtaat gctagatacc cttaataaac acatcgaact
     3001 cactaggaaa agaagtcgac ggcttcgctg ggagtgccca agaaagctac cctgccctcg
     3061 gccatcagaa ggatcttgtc aaagagctca aacagctcgg aagacggctg atgaatggtc
     3121 aggatgacgg tcttgccctt ctgcgacagc ttcttcagca cctggacgac gctgtgggcg
     3181 gtaaatgagt ccagtccgga ggtgggctca tcgcagatca gaagcggcgg atcggttagt
     3241 gcctcggagg cgaatgccag acgcttcctt tctccgccgg acagaccttt caccctgccg
     3301 ggcacaccga tgatcgtgtg ctgacatttg ctgagcgaaa gctcctggat cacctgatcc
     3361 acgcgggcca ctcgctgccg ataggtcaga tgtcgtggca tccgcaccat ggcctggaaa
     3421 atcaggtgtt ccctggccgt tagggagccg ataaagaggt catcctgctg gacataggcg
     3481 cacctggcct gcatctcctt ggcgtccaca ggttggccat tgagcagtcg catcccggat
     3541 ggcgatactt ggatgccctg cggcgatcga aaggcaaggg cattcagcag ggtcgtcttt
     3601 ccggcaccgg aactgcccat cacggccaaa agttcgcccg gataggccac gccgcaaact
     3661 gagtttcaaa ttggtaattg gaccctttat taagatttca cacagatcag ccgactgcga
     3721 atagaaactc accgttcttg agcaaatgtt tcctgggcgc cggtatgtgt cgctcgttgc
     3781 agaatagtcc gcgtgtccgg ttgaccagct gccgccatcc ggagcccggc tgattgaccg
     3841 ccccaaagat gtccatattg tgccaggcat aggtgaggtt ctcggctagt tggccgctcc
     3901 ctgaaccgga gtcctccggc ggactgggtg gcaggagcgt gccgtagttt ttggcctgcc
     3961 cgaagccctg gttaatgcag ctctgcgaag ccgctccgct gtcaccctgc aatgataggg
     4021 gatctcaaat atcaactaca agcgttatgc tcatctaacc ccgaacaaaa cgaagtatcc
     4081 tacgaagtag gtttatactt ttatttattt tttgtgcatc taggatcagc ttaaaatatc
     4141 tggttgttat attttttgta aaaaagaatg tagtcgaaaa tgaatgcctt tagatgtctt
     4201 gatcatgata tgatcttaaa aattgtctta tatagcgagc acagctacca gaataatctg
     4261 tttcgtgtca ctatttgttt gtgcgattgc ggtttgggat ttttgtgggt cgcagttctc
     4321 acgccgcaga caatttgatg ttgcaatcgc agttcctata gatcaagtga acttaagatg
     4381 tatgcacatg tactactcac attgttcaga tgctcggcag atgggtgttt gctgcctccg
     4441 cgaattaata gctcctgatc ctcttggccc attgccggga tttttcacac tttcccctgc
     4501 ttacccaccc aaaaccaatc accaccccaa tcactcaaaa aacaaacaaa aataagaagc
     4561 gagaggagtt ttggcacagc actttgtgtt taattgatgg cgtaaaccgc ttggagcttc
     4621 gtcacgaaac cgctgacaaa gtgcaactga aggcggacat tgacgctagg taacgctaca
     4681 aacggtggcg aaagagatag cggacgcagc ggcgaaagag acggcgatat ttctgtggac
     4741 agagaaggag gcaaacagcg ctgactttga gtggaatgtc attttgagtg agaggtaatc
     4801 gaaagaacct ggtacttcaa atacccttgg atcgaagtaa atttaaaact gatcagataa
     4861 gttcaatgat gtccagtgca gtaaaaaata aaaaaaaaat atgttttttt aaatctacat
     4921 tctccaaaaa agggttttat taacttacat acatactaag gccttctagt ggatccgatc
     4981 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     5041 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     5101 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     5161 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatctct
     5221 agtcaaggca ctatacatca aatattcctt attaacccct ttacaaatta aaaagctaaa
     5281 ggtacacaat ttttgagcat agttattaat agcagacact ctatgcctgt gtggagtaag
     5341 aaaaaacagt atgttatgat tataactgtt atgcctactt ataaaggtta cagaatattt
     5401 ttccataatt ttcttgtata gcagtgcagc tttttccttt gtggtgtaaa tagcaaagca
     5461 agcaagagtt ctattactaa acacagcatg actcaaaaaa cttagcaatt ctgaaggaaa
     5521 gtccttgggg tcttctacct ttctcttctt ttttggagga gtagaatgtt gagagtcagc
     5581 agtagcctca tcatcactag atggcatttc ttctgagcaa aacaggtttt cctcattaaa
     5641 ggcattccac cactgctccc attcatcagt tccataggtt ggaatctaaa atacacaaac
     5701 aattagaatc agtagtttaa cacattatac acttaaaaat tttatattta ccttagagct
     5761 ttaaatctct gtaggtagtt tgtccaatta tgtcacacca cagaagtaag gttccttcac
     5821 aaagatcctc tagaggtacc ctcgagccgc ggccgcagat ctgttaacga attcggctgt
     5881 actagtcgcg ctcgcgcgac tgacggtcgt aagcacccgc gtacgtgtcc accccggtca
     5941 caaccccttg tgtcatgtcg gcgaccctac gcccccaact gagagaactc aaaggttacc
     6001 ccagttgggg cactactccc gaaaaccgct tctgacctgg gaaaacgtga agccccgggg
     6061 catccgctga gggttgccgc cggggcttcg gtgtgtccgt cagtactagt cgaagccgaa
     6121 ttcccaattc cctattcaga gttctcttct tgtattcaat aattacttct tggcagattt
     6181 cagtagttgc agttgattta cttggttgct ggttactttt aattgattca ctttaacttg
     6241 cactttactg cagattgttt agcttgttca gctgcgcttg tttatttgct tagctttcgc
     6301 ttagcgacgt gttcactttg cttgtttgaa ttgaattgtc gctccgtaga cgaagcgcct
     6361 ctatttatac tccggcgctc gctagagtct ccgctcggag gacagtactc cgctcggagg
     6421 acagtactcc gctcggagga cagtactccg ctcggaggac agtactccgc tcggaggaca
     6481 gtactccgac ctgcaggcat gcaagcttgg atccgagctc atgcagaagc tttgcgtact
     6541 cgcaaattat taaaaataaa actttaaaaa taatttcgtc taattaatat tatgagttaa
     6601 ttcaaacccc acggacatgc taagggttaa tcaacaatca tatcgctgtc tcactcagac
     6661 tcaatacgac actcagaata ctattccttt cactcgcact tattgcaagc atacgttaag
     6721 tggatgtctc ttgccgacgg gaccacctta tgttatttca tcatggtctg gccattctca
     6781 tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga cgcaaggagt
     6841 agccgacata tatccgaaat aactgcttgt tttttttttt accattatta ccatcgtgtt
     6901 tactgtttat tgccccctca aaaagctaat gtaattatat ttgtgccaat aaaaacaaga
     6961 tatgacctat agaatacaag tatttcccct tcgaacatcc ccacaagtag actttggatt
     7021 tgtcttctaa ccaaaagact tacacacctg cataccttac atcaaaaact cgtttatcgc
     7081 tacataaaac accgggatat attttttata tacatacttt tcaaatcgcg cgccctcttc
     7141 ataattcacc tccaccacac cacgtttcgt agttgctctt tcgctgtctc ccacccgctc
     7201 tccgcaacac attcaccttt tgttcgacga ccttggagcg actgtcgtta gttccgcgcg
     7261 attcggtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     7321 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     7381 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     7441 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     7501 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7561 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7621 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7681 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7741 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7801 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7861 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     7921 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7981 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8041 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8101 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8161 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8221 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8281 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8341 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8401 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8461 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8521 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8581 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8641 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8821 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     8881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9061 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     9121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccttc
     9301 ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc
     9361 cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt
     9421 agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt
     9481 aacttctccg ccgccgatct gccgctggac tacgtgggtc tggcc
//
