LOCUS       sequence-77810-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal yEGFP tagged gene 
            sequences into the S. pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-cyEGFP
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..107
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     99..116
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     100..116
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(169..885)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(172..885)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
       61 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
      121 tcataacttc gtatataata ccatatacga agttatcccg ggagatcttt atttgtacaa
      181 ttcatccata ccatgggtaa taccagcagc agtaacaaat tctaacaaga ccatgtggtc
      241 tctcttttcg tttggatctt tggataaggc agattgagtg gataagtaat ggttgtctgg
      301 taacaagact ggaccatcac caattggagt attttgttga taatggtcag ctaattgaac
      361 agaaccatct tcaatgttgt gtctaatttt gaagttaact ttgataccat tcttttgttt
      421 gtcagccatg atgtaaacat tgtgagagtt atagttgtat tccaatttgt gacctaaaat
      481 gttaccatct tctttaaaat caataccttt taattcgatt ctattaacta aggtatcacc
      541 ttcaaacttg acttcagctc tggtcttgta gttaccgtca tctttgaaaa aaatagttct
      601 ttcttgaaca taaccttctg gcatggcaga cttgaaaaag tcatgttgtt tcatgtgatc
      661 tgggtatcta gaaaaacatt gaacaccata agttaaagta gtgactaagg ttggccatgg
      721 aactggcaat ttaccagtag tacaaataaa ttttaaggtc aatttaccgt aagtagcatc
      781 accttcacct tcaccggaga cagaaaattt gtgaccatta acatcaccat ctaattcaac
      841 caaaattggg acaacaccag tgaataattc ttcaccttta gacatagatc tacccatcga
      901 ccccgtggag ccggttgatc ctgtagaacc agtacta
//
