LOCUS       sequence-77811-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal HA-auxin-inducible 
            degron tagged gene sequences into the S. pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-cHAIAA17
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..107
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     99..116
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     100..116
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(163..852)
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
       61 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
      121 tcataacttc gtatataata ccatatacga agttatcccg ggtcaagctc tgctcttgca
      181 cttctccatc gccctcggag cgagaccaat ggcatccgat cctttcatga gacgtaaacg
      241 cttgcatgta tcgacgaaca ttggccaagg aacgtcgccg acgagcatcc aatcaccgtc
      301 tttgtcttca taagagggaa catagtccca gctattcacc aaatccatca atttcctctc
      361 attcatgaag tctatcattc cttcttctcc tccatgtttg cccatggtaa aagagctgaa
      421 catgttggac aaagcattag aaagctcatc gtagctttta tacatcctca aatcgatttt
      481 cctcaagtac ggtgctccgt ccattgatac cttcacgaac gccgccgcct ccgggccacc
      541 gcttgatttt tggcaggaaa ccatcacgtt cttccggtat gatctcaccg gtggccatcc
      601 cacaacttgt gccttggccg gaggtttggc tggatcttta ggacaagcac tcttctcctt
      661 ggaatcaaaa gtcacgacgt catgagtcgt agatccttcc ttgtttgcag gctcattatt
      721 cagatttagc ttcagatcaa ccgtctctga gaaccctctc ttgtttccgg ttaccggagc
      781 cactgtatct ccaccgggaa gaccaagaca cagctcagtc tccctcagat tcagctcgac
      841 actgcccatc atagatctac ccatcgaccc cgtggagccg gttgatcctg tagaaccagt
      901 actagaagca taatc
//
