LOCUS       sequence-77813-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal yEGFP tagged gene 
            sequences together with 6 copies of the core DSR motif into the S. 
            pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-cyEGFP-6XDSR
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..107
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     99..116
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     100..116
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
       61 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
      121 tcataacttc gtatataata ccatatacga agttatcccg gggtttaaca ttacgtttaa
      181 cctgccgttt aaagttctgt ttaagaatgg gtttaatacg gagtttaaag atctttattt
      241 gtacaattca tccataccat gggtaatacc agcagcagta acaaattcta acaagaccat
      301 gtggtctctc ttttcgtttg gatctttgga taaggcagat tgagtggata agtaatggtt
      361 gtctggtaac aagactggac catcaccaat tggagtattt tgttgataat ggtcagctaa
      421 ttgaacagaa ccatcttcaa tgttgtgtct aattttgaag ttaactttga taccattctt
      481 ttgtttgtca gccatgatgt aaacattgtg agagttatag ttgtattcca atttgtgacc
      541 taaaatgtta ccatcttctt taaaatcaat accttttaat tcgattctat taactaaggt
      601 atcaccttca aacttgactt cagctctggt cttgtagtta ccgtcatctt tgaaaaaaat
      661 agttctttct tgaacataac cttctggcat ggcagacttg aaaaagtcat gttgtttcat
      721 gtgatctggg tatctagaaa aacattgaac accataagtt aaagtagtga ctaaggttgg
      781 ccatggaact ggcaatttac cagtagtaca aataaatttt aaggtcaatt taccgtaagt
      841 agcatcacct tcaccttcac cggagacaga aaatttgtga ccattaacat caccatctaa
      901 ttcaaccaaa attgggacaa caccagtgaa taattcttca c
//
