LOCUS       sequence-77815-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-EGFP
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(222..255)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gggggcgtga attttcgctg cttgtccttt tcctgctggt tgctcccatt cttaggtgaa
       61 tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta
      121 atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat
      181 gcccaattga gatccgctag tgaacctctt cgagggacct aataacttcg tatagcatac
      241 attatacgaa gttatattaa gggttccggc aattgcccca tgttgggagg acgaaaatgg
      301 tgacaagaca gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat
      361 tctttagtgc gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta
      421 catcactcct gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg
      481 tcatcaccct ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact
      541 ccatcgtcaa agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata
      601 acaaggtcct taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc
      661 atgaaataac agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc
      721 attccaaagg ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt
      781 cctcgcctta ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc
      841 caagttcctt cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct
      901 tctgcaatgt tctcaaattt cgggttggaa cctccttgac cacgatgctt tccaaaccac
      961 cctccttttt
//
