LOCUS       sequence-77816-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mRuby2-Tub1+3'UTR::LEU2
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             612..620
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctcgtacgct gcaggtcgac cggcccagtt tttgaacaca tagttctttc taaatgggat
       61 agtagacatg gggtgaaaaa cctgataata tcggtaactt taattttacg ggttcttgtt
      121 ttaccttcta atgaatttta agttttgaaa agcagaaaat cagatattga aactatagat
      181 ctgcttacct tgaagttcac tatttttgga attctccttg ctaatagtta cttctactct
      241 tgctttgtcc tggatatcaa tggaagatgt cctcatttcc attttttttt tcaattcgac
      301 ggcaaggcgg gagaaaagga actagggata caaaaatgca attactaatg aaatccgtga
      361 aataatcatg gaaatgggtg atgtaagaat ctgatgaaac aagtacaaat gtctttggcc
      421 catcaactac gatgagactt actaagcttt catcaaatgc aggttagaga aggagaatgg
      481 tttttttttt tattttttgt acatagatat tagagaaata taataagtag attatagata
      541 agatatcacc aaagagatat aacagtaggg ggaatttttt tttttacaat atacttgaaa
      601 tatagaaagg ataaggaggt tgggggcgag agtgaacctt aaaattcctc ttcctcagcg
      661 tatgagtcgg cacccacttc gatgtaatct ctttctaaag cagccaaatc ttctctagct
      721 tcggtgaatt caccttcttc cataccttca ccgacatacc agtggacgaa agcacgtttg
      781 gcatacatta aatcgaattt tctatcgatt ctcttccaag cctcagcaat ggatgtggta
      841 tttgacaaca tacagacgg
//
