LOCUS       sequence-77826-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mRuby2-Tub1+3'UTR::TRP1
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             608..616
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tacgctgcag gtcgaccggc ccagtttttg aacacatagt tctttctaaa tgggatagta
       61 gacatggggt gaaaaacctg ataatatcgg taactttaat tttacgggtt cttgttttac
      121 cttctaatga attttaagtt ttgaaaagca gaaaatcaga tattgaaact atagatctgc
      181 ttaccttgaa gttcactatt tttggaattc tccttgctaa tagttacttc tactcttgct
      241 ttgtcctgga tatcaatgga agatgtcctc atttccattt tttttttcaa ttcgacggca
      301 aggcgggaga aaaggaacta gggatacaaa aatgcaatta ctaatgaaat ccgtgaaata
      361 atcatggaaa tgggtgatgt aagaatctga tgaaacaagt acaaatgtct ttggcccatc
      421 aactacgatg agacttacta agctttcatc aaatgcaggt tagagaagga gaatggtttt
      481 tttttttatt ttttgtacat agatattaga gaaatataat aagtagatta tagataagat
      541 atcaccaaag agatataaca gtagggggaa tttttttttt tacaatatac ttgaaatata
      601 gaaaggataa ggaggttggg ggcgagagtg aaccttaaaa ttcctcttcc tcagcgtatg
      661 agtcggcacc cacttcgatg taatctcttt ctaaagcagc caaatcttct ctagcttcgg
      721 tgaattcacc ttcttccata ccttcaccga cataccagtg gacgaaagca cgtttggcat
      781 acattaaatc gaattttcta tcgattctct tccaagcctc agcaatggat gtggtatttg
      841 acaacataca gacggcccta tccacagtgg ccaattgtga gtttggtgtg gcagttggag
      901 gttcgta
//
