LOCUS       sequence-77827-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mEos2-Tub1+3'UTR::TRP1
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             610..618
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cgtacgctgc aggtcgaccg gcccagtttt tgaacacata gttctttcta aatgggatag
       61 tagacatggg gtgaaaaacc tgataatatc ggtaacttta attttacggg ttcttgtttt
      121 accttctaat gaattttaag ttttgaaaag cagaaaatca gatattgaaa ctatagatct
      181 gcttaccttg aagttcacta tttttggaat tctccttgct aatagttact tctactcttg
      241 ctttgtcctg gatatcaatg gaagatgtcc tcatttccat tttttttttc aattcgacgg
      301 caaggcggga gaaaaggaac tagggataca aaaatgcaat tactaatgaa atccgtgaaa
      361 taatcatgga aatgggtgat gtaagaatct gatgaaacaa gtacaaatgt ctttggccca
      421 tcaactacga tgagacttac taagctttca tcaaatgcag gttagagaag gagaatggtt
      481 ttttttttta ttttttgtac atagatatta gagaaatata ataagtagat tatagataag
      541 atatcaccaa agagatataa cagtaggggg aatttttttt tttacaatat acttgaaata
      601 tagaaaggat aaggaggttg ggggcgagag tgaaccttaa aattcctctt cctcagcgta
      661 tgagtcggca cccacttcga tgtaatctct ttctaaagca gccaaatctt ctctagcttc
      721 ggtgaattca ccttcttcca taccttcacc gacataccag tggacgaaag cacgtttggc
      781 atacattaaa tcgaattttc tatcgattct cttccaagcc tcagcaatgg atgtggtatt
      841 tgacaacata cagacggccc tatccacagt ggccaattgt gagtttggtg tggcagttgg
      901 aggttcgtag caaataccga tcttgaaacc agttggac
//
