LOCUS       sequence-77828-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mTurquoise2-Tub1+3'UTR::HIS3
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             604..612
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctgcaggtcg accggcccag tttttgaaca catagttctt tctaaatggg atagtagaca
       61 tggggtgaaa aacctgataa tatcggtaac tttaatttta cgggttcttg ttttaccttc
      121 taatgaattt taagttttga aaagcagaaa atcagatatt gaaactatag atctgcttac
      181 cttgaagttc actatttttg gaattctcct tgctaatagt tacttctact cttgctttgt
      241 cctggatatc aatggaagat gtcctcattt ccattttttt tttcaattcg acggcaaggc
      301 gggagaaaag gaactaggga tacaaaaatg caattactaa tgaaatccgt gaaataatca
      361 tggaaatggg tgatgtaaga atctgatgaa acaagtacaa atgtctttgg cccatcaact
      421 acgatgagac ttactaagct ttcatcaaat gcaggttaga gaaggagaat ggtttttttt
      481 tttatttttt gtacatagat attagagaaa tataataagt agattataga taagatatca
      541 ccaaagagat ataacagtag ggggaatttt tttttttaca atatacttga aatatagaaa
      601 ggataaggag gttgggggcg agagtgaacc ttaaaattcc tcttcctcag cgtatgagtc
      661 ggcacccact tcgatgtaat ctctttctaa agcagccaaa tcttctctag cttcggtgaa
      721 ttcaccttct tccatacctt caccgacata ccagtggacg aaagcacgtt tggcatacat
      781 taaatcgaat tttctatcga ttctcttcca agcctcagca atggatgtgg tatttgacaa
      841 catacagacg gc
//
