LOCUS       sequence-77832-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mRuby2-Tub1+3'UTR::HIS3
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             613..621
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cttcgtacgc tgcaggtcga ccggcccagt ttttgaacac atagttcttt ctaaatggga
       61 tagtagacat ggggtgaaaa acctgataat atcggtaact ttaattttac gggttcttgt
      121 tttaccttct aatgaatttt aagttttgaa aagcagaaaa tcagatattg aaactataga
      181 tctgcttacc ttgaagttca ctatttttgg aattctcctt gctaatagtt acttctactc
      241 ttgctttgtc ctggatatca atggaagatg tcctcatttc catttttttt ttcaattcga
      301 cggcaaggcg ggagaaaagg aactagggat acaaaaatgc aattactaat gaaatccgtg
      361 aaataatcat ggaaatgggt gatgtaagaa tctgatgaaa caagtacaaa tgtctttggc
      421 ccatcaacta cgatgagact tactaagctt tcatcaaatg caggttagag aaggagaatg
      481 gttttttttt ttattttttg tacatagata ttagagaaat ataataagta gattatagat
      541 aagatatcac caaagagata taacagtagg gggaattttt ttttttacaa tatacttgaa
      601 atatagaaag gataaggagg ttgggggcga gagtgaacct taaaattcct cttcctcagc
      661 gtatgagtcg gcacccactt cgatgtaatc tctttctaaa gcagccaaat cttctctagc
      721 ttcggtgaat tcaccttctt ccataccttc accgacatac cagtggacga aagcacgttt
      781 ggcatacatt aaatcgaatt ttctatcgat tctcttccaa gcctcagcaa tggatgtggt
      841 atttgacaac atacagacgg ccctatccac agtggccaat tgtgagtttg gtgt
//
