LOCUS       sequence-77833-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mEos2-Tub1+3'UTR::HIS3
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             607..615
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acgctgcagg tcgaccggcc cagtttttga acacatagtt ctttctaaat gggatagtag
       61 acatggggtg aaaaacctga taatatcggt aactttaatt ttacgggttc ttgttttacc
      121 ttctaatgaa ttttaagttt tgaaaagcag aaaatcagat attgaaacta tagatctgct
      181 taccttgaag ttcactattt ttggaattct ccttgctaat agttacttct actcttgctt
      241 tgtcctggat atcaatggaa gatgtcctca tttccatttt ttttttcaat tcgacggcaa
      301 ggcgggagaa aaggaactag ggatacaaaa atgcaattac taatgaaatc cgtgaaataa
      361 tcatggaaat gggtgatgta agaatctgat gaaacaagta caaatgtctt tggcccatca
      421 actacgatga gacttactaa gctttcatca aatgcaggtt agagaaggag aatggttttt
      481 ttttttattt tttgtacata gatattagag aaatataata agtagattat agataagata
      541 tcaccaaaga gatataacag tagggggaat tttttttttt acaatatact tgaaatatag
      601 aaaggataag gaggttgggg gcgagagtga accttaaaat tcctcttcct cagcgtatga
      661 gtcggcaccc acttcgatgt aatctctttc taaagcagcc aaatcttctc tagcttcggt
      721 gaattcacct tcttccatac cttcaccgac ataccagtgg acgaaagcac gtttggcata
      781 cattaaatcg aattttctat cgattctctt ccaagcctca gcaatggatg tggtatttga
      841 caacatacag acggccctat ccacagtgg
//
