LOCUS       sequence-77850-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. Coli N-term HexHis Tagged Protein Expression Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Bower BD, Griffith JD
  TITLE     TRF1 and TRF2 differentially modulate Rad51-mediated telomeric and 
            nontelomeric displacement loop formation in vitro.
  JOURNAL   Biochemistry. 2014 Sep 2;53(34):5485-95. doi: 10.1021/bi5006249. 
            Epub 2014 Aug 21.
  PUBMED    25115914
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi5006249";
            journalName: "Biochemistry"; date: "2014-09-2- 2"; volume: "53"; 
            issue: "34"; pages: "5485-95"
COMMENT     Sequence Label: pTRCHisB-TRF2∆M
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..124
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             140..157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             161..193
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             206..226
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggcactgctc tttaacaatt tatcagacaa tctgtgtggg cactcgaccg gaattatcga
       61 ttaactttat tattaaaaat taaagaggta tatattaatg tatcgattaa ataaggagga
      121 ataaaccatg gggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg
      181 acagcaaatg ggtcgggatc tgtacgaaaa cctgtatttt cagggcgatc cgagctcgag
      241 atctgcgcgc gatcgatcgg gaggaggcgg gagtagcgac ggcagcgggc gggcagctgg
      301 caggcgggcg tcccgcagta gcgggcgggc ccggcggggg cgccacgagc cggggctggg
      361 gggcccggcg gagcgcggcg cgggggaggc acggctggaa gaggcagtca atcgctgggt
      421 gctcaagttc tacttccacg aggcgctgcg ggcctttcgg ggtagccggt acggggactt
      481 cagacagatc cgggacatca tgcaggcttt gcttgtcagg cccttgggga aggagcacac
      541 cgtgtcccga ttgctgcggg ttatgcagtg tctgtcgcgg attgaagaag gggaaaattt
      601 agactgttcc tttgatatgg aggctgagct cacaccactg gaatcagcta tcaatgtgct
      661 ggagatgatt aaaacggaat ttacactgac agaagcagtg gtcgaatcca gtagaaaact
      721 ggtcaaggaa gctgctgtca ttatttgtat caaaaacaaa gaatttgaaa aggcttcaaa
      781 aattttgaaa aaacatatgt ccaaggaccc cacaactca
//
