LOCUS       sequence-77856-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express human erythropoietin using lentiviral delivery system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Lin RZ, Dreyzin A, Aamodt K, Li D, Jaminet SC, Dudley AC, 
            Melero-Martin JM
  TITLE     Induction of erythropoiesis using human vascular networks 
            genetically engineered for controlled erythropoietin release.
  JOURNAL   Blood. 2011 Nov 17;118(20):5420-8. doi: 
            10.1182/blood-2011-08-372946. Epub 2011 Sep 21.
  PUBMED    21937702
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2011-08-372946"; journalName: "Blood"; date: 
            "2011-11-17- 17"; volume: "118"; issue: "20"; pages: "5420-8"
COMMENT     Sequence Label: pLenti6.3-hEPO
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..88)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    141..165
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(781..805)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgatc attactaacc ggtacgcgta gaatcgagac
       61 cgaggagagg gttagggata ggcttacctt cgaaccgcgg gccctctaga ctcgagcggc
      121 cgccactgtg ctggatatca accactttgt acaagaaagc tgggttgcgg ccgcactcga
      181 gctatctgtc ccctgtcctg caggcctccc ctgtgtacag cttcagcttt ccccggagga
      241 aattggagta gactcggaag agtttgcgga aagtgtcagc agtgattgtt cggagtggag
      301 cagctgaggc cgcatctgga ggggagatgg cttccttctg ggctcccaga gcccgaagca
      361 gagtggtgag gctgcgaagg ccactgacgg ctttatccac atgcagctgc aggggctccc
      421 acggctggga agagttgacc aacagggcct ggccccgcag gacagcttcc gacagcaggg
      481 ccaggccctg ccagacttct acggcctgct gcccgacctc catcctcttc caggcataga
      541 aattaacttt ggtgtctggg acagtgatat tctcattcaa gctgcagtgt tcagcacagc
      601 ccgtcgtgat attctcggcc tccttggcct ccaagaggta cctctccagg actcggctgt
      661 cacagatgag gcgtggtggg gcgcccagga ctgggaggcc cagagggagc gacagcaggg
      721 acaggagaag ccacagccag gcaggacatt cgtgcacccc catggttcga actccttcca
      781 agcctgcttt tttgtacaaa cttgttgata tctgcagaat tccaccacac tggactagtg
      841 gatcctctag agtcggtgtc ttctatggag gtcaaaacag cgtggatggc gtctcc
//
