LOCUS       sequence-77857-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express human erythropoietin under the control of 
            tetracycline-regulatable CMV promoter using lentiviral delivery 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Lin RZ, Dreyzin A, Aamodt K, Li D, Jaminet SC, Dudley AC, 
            Melero-Martin JM
  TITLE     Induction of erythropoiesis using human vascular networks 
            genetically engineered for controlled erythropoietin release.
  JOURNAL   Blood. 2011 Nov 17;118(20):5420-8. doi: 
            10.1182/blood-2011-08-372946. Epub 2011 Sep 21.
  PUBMED    21937702
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2011-08-372946"; journalName: "Blood"; date: 
            "2011-11-17- 17"; volume: "118"; issue: "20"; pages: "5420-8"
COMMENT     Sequence Label: pLenti4/TO-hEPO
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..57)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    110..134
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(750..774)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    815..833
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    836..854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(856..955)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(885..905)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttactaaccg gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc
       61 gaaccgcggg ccctctagac tcgagcggcc gccactgtgc tggatatcaa ccactttgta
      121 caagaaagct gggttgcggc cgcactcgag ctatctgtcc cctgtcctgc aggcctcccc
      181 tgtgtacagc ttcagctttc cccggaggaa attggagtag actcggaaga gtttgcggaa
      241 agtgtcagca gtgattgttc ggagtggagc agctgaggcc gcatctggag gggagatggc
      301 ttccttctgg gctcccagag cccgaagcag agtggtgagg ctgcgaaggc cactgacggc
      361 tttatccaca tgcagctgca ggggctccca cggctgggaa gagttgacca acagggcctg
      421 gccccgcagg acagcttccg acagcagggc caggccctgc cagacttcta cggcctgctg
      481 cccgacctcc atcctcttcc aggcatagaa attaactttg gtgtctggga cagtgatatt
      541 ctcattcaag ctgcagtgtt cagcacagcc cgtcgtgata ttctcggcct ccttggcctc
      601 caagaggtac ctctccagga ctcggctgtc acagatgagg cgtggtgggg cgcccaggac
      661 tgggaggccc agagggagcg acagcaggga caggagaagc cacagccagg caggacattc
      721 gtgcaccccc atggttcgaa ctccttccaa gcctgctttt ttgtacaaac ttgttgatat
      781 ctgcagaatt ccaccacact ggactagtcg acgatctcta tcactgatag ggagatctct
      841 atcactgata gggagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      901 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
      961 aacaaactcc cattgacgtc a
//
