LOCUS       sequence-77862-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Left TALEN for PKP2 with NN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga 
            PP, So PL, Conklin BR
  TITLE     Isolation of single-base genome-edited human iPS cells without 
            antibiotic selection.
  JOURNAL   Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 
            2014 Feb 9.
  PUBMED    24509632
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2840"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "291-3"
COMMENT     Sequence Label: MR015-PKP2 Left TALEN_NN
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..165
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             172..192
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gccaccatgg actacaaaga ccatgacggt
      121 gattataaag atcatgacat cgattacaag gatgacgatg acaagatggc ccccaagaag
      181 aagaggaagg tgggcattca ccgcggggta cctatggtgg acttgaggac actcggttat
      241 tcgcaacagc aacaggagaa aatcaagcct aaggtcagga gcaccgtcgc gcaacaccac
      301 gaggcgcttg tggggcatgg cttcactcat gcgcatattg tcgcgctttc acagcaccct
      361 gcggcgcttg ggacggtggc tgtcaaatac caagatatga ttgcggccct gcccgaagcc
      421 acgcacgagg caattgtagg ggtcggtaaa cagtggtcgg gagcgcgagc acttgaggcg
      481 ctgctgactg tggcgggtga gcttaggggg cctccgctcc agctcgacac cgggcagctg
      541 ctgaagatcg cgaagagagg gggagtaaca gcggtagagg cagtgcacgc ctggcgcaat
      601 gcgctcaccg gtgcccccct gaacctgacc ccggaccaag tggtggctat cgccagcaac
      661 aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
      721 catggcctga ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg
      781 ctcgaaacgg tgcagcggct gttg
//
