LOCUS       sequence-77882-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Venus and FLAG-tagged human CD59, a GPI-AP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Rivier AS, Castillon GA, Michon L, Fukasawa M, Romanova-Michaelides 
            M, Jaensch N, Hanada K, Watanabe R
  TITLE     Exit of GPI-anchored proteins from the ER differs in yeast and 
            mammalian cells.
  JOURNAL   Traffic. 2010 Aug;11(8):1017-33. doi: 
            10.1111/j.1600-0854.2010.01081.x. Epub 2010 May 11.
  PUBMED    20477992
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2010.01081.x"; journalName: "Traffic"; date: 
            "2010-08"; volume: "11"; issue: "8"; pages: "1017-33"
COMMENT     Sequence Label: pME-puro-Venus-FLAG-CD59
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(403..426)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(476..496)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
       61 gttttttctc tagactagtc tagcggccgc ttagggatga aggctccagg ctgctgccag
      121 aaatggagtc accagcagaa gaactgtttt ctctgataag gatgtcccac cattctcgag
      181 ctgttcgtta aagttacaca ggtccttctt gcagcagtag tacgttagct cattttccct
      241 caagcgggtt gtgacgtcgt tgaaattgca atgctcaaac ttccaacact tgttatacac
      301 ttgtaaccca gctttggtaa tgagacacgc atcaaaatca gatgaacaat tgacggctgt
      361 tttgcagtca gcagttgggt taggacagtt gtagcactga agcttgtcat cgtcgtcctt
      421 gtagtcctgc agggctttgt atagttcatc catgccatgt gtaatcccag cagctgttac
      481 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg
      541 ataggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
      601 ttgataatgg tctgctagtt gaacacctcc atcttcaatg ttgtgtctaa ttttgaagtt
      661 agctttgatt ccattctttt gtttgtctgc agtgatgtat acattgtgtg agttatagtt
      721 gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cttttaactc
      781 gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct tgtagttccc
      841 gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg cactcttgaa
      901 aaagtcatgc cgtttcatat gatctgggta tctagcaaag cacatta
//
