LOCUS       sequence-77883-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  TITLE     pME-hygro-SNAP-FLAG-CD59
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pME-hygro-SNAP-FLAG-CD59
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..647
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             657..680
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctgcggaa ttccaccatg ggaatccaag gagggtctgt cctgttcggg ctgctgctcg
       61 tcctggctgt cttctgccat tcaggtcata gcctgcaggg catggacaaa gactgcgaaa
      121 tgaagcgcac caccctggat agccctctgg gcaagctgga actgtctggg tgcgaacagg
      181 gcctgcaccg tatcatcttc ctgggcaaag gaacatctgc cgccgacgcc gtggaagtgc
      241 ctgccccagc cgccgtgctg ggcggaccag agccactgat gcaggccacc gcctggctca
      301 acgcctactt tcaccagcct gaggccatcg aggagttccc tgtgccagcc ctgcaccacc
      361 cagtgttcca gcaggagagc tttacccgcc aggtgctgtg gaaactgctg aaagtggtga
      421 agttcggaga ggtcatcagc tacagccacc tggccgccct ggccggcaat cccgccgcca
      481 ccgccgccgt gaaaaccgcc ctgagcggaa atcccgtgcc cattctgatc ccctgccacc
      541 gggtggtgca gggcgacctg gacgtggggg gctacgaggg cgggctcgcc gtgaaagagt
      601 ggctgctggc ccacgagggc cacagactgg gcaagcctgg gctgggtgcc ctgcaggact
      661 acaaggacga cgatgacaag cttcagtgct acaactgtcc taacccaact gctgactgca
      721 aaacagccgt caattgttca tctgattttg atgcgtgtct cattaccaaa gctgggttac
      781 aagtgtataa caagtgttgg aagtttgagc attgcaattt caacgacgtc acaacccgct
      841 tgagggaaaa tgagctaacg tactactgct gcaagaagga cctgtgtaac tttaacgaac
      901 agctcgagaa tggtgggac
//
