LOCUS       sequence-77889-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag
            at its N-terminus and Myc tag at its C-terminus by co-transfection 
            with psPAX2 and pMD2G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pSLIK-NFLAG-hEWSR1G511A-CMyc
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    90..114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    join(153..171,172..194,195..213,214..236,237..255,256..278,
                     279..297,298..320,321..339,340..362,363..381,382..404,
                     405..423)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        456..494
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     491..515
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             603..626
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
       61 ccagtttggt taattctaga cagttatcaa caagtttgta caaaaaagca ggctttaaag
      121 gaaccaattc agtcgactgg atcctttacc actccctatc agtgatagag aaaagtgaaa
      181 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      241 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      301 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      361 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      421 agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct
      481 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      541 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ctagtcacca
      601 tggactacaa ggacgacgat gacaaaatgg cgtccacgga ttacagtacc tatagccaag
      661 ctgcagcgca gcagggctac agtgcttaca ccgcccagcc cactcaagga tatgcacaga
      721 ccacccaggc atatgggcaa caaagctatg gaacctatgg acagcccact gatgtcagct
      781 atacccaggc tcagaccact gcaacctatg ggcagaccgc ctatgcaact tcttatggac
      841 agcctcccac tggttatact actccaactg ccccccaggc atacagccag cctgtccagg
      901 ggtatggcac tggtgcttat gataccacca ctgctacagt caccaccacc caggcctcct
      961 atgcagctca gtctgcatat g
//
