LOCUS       sequence-77893-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces lentivirus expressing human EWSR1P552L mutant with FLAG tag
            at its N-terminus and Myc tag at its C-terminus by co-transfection 
            with psPAX2 and pMD2G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pSLIK-NFLAG-hEWSR1P552L-CMyc
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    91..115
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    join(154..172,173..195,196..214,215..237,238..256,257..279,
                     280..298,299..321,322..340,341..363,364..382,383..405,
                     406..424)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        457..495
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     492..516
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             604..627
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
       61 tccagtttgg ttaattctag acagttatca acaagtttgt acaaaaaagc aggctttaaa
      121 ggaaccaatt cagtcgactg gatcctttac cactccctat cagtgataga gaaaagtgaa
      181 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      241 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      301 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
      361 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      421 gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta ggcgtgtacg gtgggaggcc
      481 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
      541 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga actagtcacc
      601 atggactaca aggacgacga tgacaaaatg gcgtccacgg attacagtac ctatagccaa
      661 gctgcagcgc agcagggcta cagtgcttac accgcccagc ccactcaagg atatgcacag
      721 accacccagg catatgggca acaaagctat ggaacctatg gacagcccac tgatgtcagc
      781 tatacccagg ctcagaccac tgcaacctat gggcagaccg cctatgcaac ttcttatgga
      841 cagcctccca ctggttatac tactccaact gccccccagg catacagcca gcctgtccag
      901 gggtatggca ctggtgctta tgataccacc actgctacag tcacca
//
