LOCUS       sequence-77899-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SSR1.0 has a one I-SceI target site for generate specific double 
            strand break after I-SceI endonuclease expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Gomez-Cabello D, Jimeno S, Fernandez-Avila MJ, Huertas P
  TITLE     New tools to study DNA double-strand break repair pathway choice.
  JOURNAL   PLoS One. 2013 Oct 14;8(10):e77206. doi: 
            10.1371/journal.pone.0077206.
  PUBMED    24155929
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-14"; volume: "8(10):e77206. doi"; pages: " 
            10.1371/journal.pone.0077206"
COMMENT     Sequence Label: SeeSaw Reporter (SSR1.0)
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      456..465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(507..528)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgctagcgct accggtcgcc accatggtga gcggcctgct gaaggagagt atgcgcatca
       61 agatgtacat ggagggcacc gtgaacggcc actacttcaa gtgcgagggc gagggcgacg
      121 gcaacccctt cgccggcacc cagagcatga gaatccacgt gaccgagggc gcccccctgc
      181 ccttcgcctt cgacatcctg gccccctgct gcgagtacgg cagcaggacc ttcgtgcacc
      241 acaccgccga gatccccgac ttcttcaagc agagcttccc cgagggcttc acctgggaga
      301 gaaccaccac ctacgaggac ggcggcatcc tgaccgccca ccaggacacc agcctggagg
      361 gcaactgcct gatctacaag gtgaaggtgc acggcaccaa cttccccgcc gacggccccg
      421 tgatgaagaa caagagcggt ctagcgctac cggtcgccac catggtgagc aagggcgagg
      481 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      541 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      601 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      661 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      721 ccgccatgcc cgaa
//
