LOCUS       sequence-7790-ex       10899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: pCEP4YPet-MAMM
FEATURES             Location/Qualifiers
     source          1..10899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      377..965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     866..885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1396..1541
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             1584..2621
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(2619..2638)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2663..2711
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        3365..3744
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3745..3948
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3899..3919
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3945..3969
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4009..4018
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4015..4017,4018..4020,4021..4734)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(4321..4340)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    4746..4880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4770..4789)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4824..4843
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5023..5045)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5603..7392
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(7694..9619)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(10054..10072)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10140..10244
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10245..10313,10314..10899,1..206)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10463..10482)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
       61 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      121 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      181 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
      241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
      301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
      361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
      541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      721 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      781 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      841 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      901 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      961 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct
     1021 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     1081 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
     1141 ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat
     1201 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     1261 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc
     1321 atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat
     1381 gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac
     1441 acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt
     1501 gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg
     1561 cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     1621 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     1681 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     1741 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     1801 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     1861 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     1921 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     1981 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     2041 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     2101 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     2161 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     2221 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     2281 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     2341 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     2401 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     2461 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     2521 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     2581 ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata
     2641 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     2701 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     2761 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     2821 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac
     2881 tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt
     2941 attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg
     3001 tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg
     3061 tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct
     3121 agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     3181 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     3241 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     3301 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     3361 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     3421 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3481 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3541 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3601 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     3661 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     3721 gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga
     3781 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3841 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg
     3901 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     3961 cgtcagatct ctagaagctg ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
     4021 agcaaaggcg aagagctgtt caccggcgtg gtgcccatcc tggtggagct ggacggcgac
     4081 gtgaacggcc acaagttcag cgtgagcggc gagggcgagg gcgacgccac ctacggcaag
     4141 ctgaccctga agctgctgtg caccaccggc aagctgcccg tgccctggcc caccctggtg
     4201 accaccctgg gctacggcgt gcagtgcttc gcccggtacc ccgaccacat gaagcagcac
     4261 gacttcttca agagcgccat gcccgagggc tacgtgcagg agcggaccat cttcttcaag
     4321 gacgacggca actacaagac ccgggccgag gtgaagttcg agggcgacac cctggtgaac
     4381 cggatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg ccacaagctg
     4441 gagtacaact acaacagcca caacgtgtac atcaccgccg acaagcagaa gaacggcatc
     4501 aaggccaact tcaagatccg gcacaacatc gaggacggcg gcgtgcagct ggccgaccac
     4561 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4621 agctaccaga gcgccctgtt caaggacccc aacgagaagc gggaccacat ggtgctgctg
     4681 gagttcctga ccgccgccgg catcaccgag ggcatgaacg agctctataa gtaaagcggc
     4741 caacggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     4801 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     4861 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     4921 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     4981 atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     5041 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggc
     5101 gtcagcgggt gttggcgggt gtcggggcgc agccatgagg tcgactctag aggatcgatg
     5161 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     5221 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     5281 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     5341 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     5401 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     5461 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     5521 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     5581 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     5641 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     5701 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     5761 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     5821 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     5881 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     5941 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     6001 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     6061 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     6121 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     6181 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     6241 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     6301 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     6361 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     6421 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     6481 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     6541 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     6601 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     6661 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     6721 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     6781 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     6841 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     6901 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     6961 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     7021 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     7081 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     7141 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     7201 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     7261 cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt
     7321 gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt
     7381 gtccttttcc tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc
     7441 gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg
     7501 ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag
     7561 gacgaaaatg gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac
     7621 tggggattta ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc
     7681 ctaacaagtt acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc
     7741 cgtcatctcc gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag
     7801 gcaaatctac tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg
     7861 gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt
     7921 gtaaaaagac catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg
     7981 gtccaggggc cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag
     8041 caagggcagt tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca
     8101 caccggcgac ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag
     8161 ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct
     8221 ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc
     8281 ttgggccttc tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag
     8341 gctcaccatc tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt
     8401 ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact
     8461 gactactggg actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt
     8521 cacgacttcc ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc
     8581 gaccccggcc tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc
     8641 cacctcctgc tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc
     8701 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     8761 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     8821 ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg
     8881 ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg
     8941 cccctcctgc ccctcctcct gctcctgccc ctcctgctcc tgcccctcct gcccctcctg
     9001 cccctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc
     9061 ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc
     9121 ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctcct gctcctgccc
     9181 ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc
     9241 ctgctcctgc ccctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     9301 ctcctgctcc tgcccctcct gctcctgccc ctcccgctcc tgctcctgct cctgttccac
     9361 cgtgggtccc tttgcagcca atgcaacttg gacgtttttg gggtctccgg acaccatctc
     9421 tatgtcttgg ccctgatcct gagccgcccg gggctcctgg tcttccgcct cctcgtcctc
     9481 gtcctcttcc ccgtcctcgt ccatggttat caccccctct tctttgaggt ccactgccgc
     9541 cggagccttc tggtccagat gtgtctccct tctctcctag gccatttcca ggtcctgtac
     9601 ctggcccctc gtcagacatg attcacacta aaagagatca atagacatct ttattagacg
     9661 acgctcagtg aatacaggga gtgcagactc ctgccccctc caacagcccc cccaccctca
     9721 tccccttcat ggtcgctgtc agacagatcc aggtctgaaa attccccatc ctccgaacca
     9781 tcctcgtcct catcaccaat tactcgcagc ccggaaaact cccgctgaac atcctcaaga
     9841 tttgcgtcct gagcctcaag ccaggcctca aattcctcgt cccccttttt gctggacggt
     9901 agggatgggg attctcggga cccctcctct tcctcttcaa ggtcaccaga cagagatgct
     9961 actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat cgatgataag
    10021 ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
    10081 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
    10141 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10201 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10261 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10321 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    10381 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10441 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
    10501 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10561 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10621 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10681 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10741 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
    10801 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10861 tagactggat ggaggcggat aaagttgcag gaccacttc
//
