LOCUS       sequence-77900-e       11053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expression of the site-specific recombinase Dre and BFP under the
            control of the Thy1.2 expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 11053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: Thy1.2-NLS_HA_Dre-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..11053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4176..4196
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4197..4223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4230..5252
                     /product="site-specific recombinase"
                     /label=Dre recombinase
                     /note="Dre is a site-specific recombinase from 
                     bacteriophage D6. Recombination occurs at rox sequences."
     CDS             5262..5318
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5319..6017
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     primer_bind     complement(8806..8825)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8925..8947
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8985..9003)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9071..9175
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9176..9244,9245..10036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9394..9413)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10207..10795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10696..10715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10949..10966
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgagctcgaa ttcagagacc gggaaccaaa ctagccttta aaaaacataa gtacaggagc
       61 cagcaagatg gctcagtggg taaaggtgcc taccagcaag cctgacagcc tgagttcagt
      121 ccccacgaac tacgtggtag gagaggacca accaactctg gaaatctgtt ctgcaaacac
      181 atgctcacac acacacacac aaatagtata aacaatttta aatttcattt aaaaataatt
      241 tgtaaacaaa atcattagca caggttttag aaagagcctc ttggtgacat caagttgatg
      301 ctgtagatgg ggtatcattc ctgaggaccc aaaaccgggt ctcagccttt ccccattctg
      361 agagttctct cttttctcag ccactagctg aagagtagag tggctcagca ctgggctctt
      421 gagttcccaa gtcctacaac tggtcagcct gactactaac cagccatgaa gaaacaagga
      481 gtggatgggc tgagtctgct gggatgggag tggagttagt aagtggccat ggatgtaatg
      541 accccagcaa tgctggctag aaggcatgcc tcctttcctt gtctggagac ggaacgggat
      601 catcttgtac tcacagaagg gagaacattc tagctggttg ggccaaaatg tgcaagttca
      661 cctggaggtg gtggtgcatg cttttaactc cagtactcag gaggcagggc caggtggatc
      721 tctgtgagtt caagaccagc ctgcactatg gagagagttt tgggacagcc agagttacac
      781 agaaaaatcc tggtggaaaa tctgaaagaa agagagaaag aaagaaagaa agaaagaaag
      841 aaagaaagaa agaaagaaag aaagaaagaa agaaagaaag gaagaaagaa agaaagagtg
      901 gcaggcaggc aggcaggagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg
      961 aaggaaggaa ggaaggaagg aaaataggtg cgacttcaag atccggagtt acaagcagaa
     1021 tgcactgttt ccctaacagg gccaagtgtt ttgagtaact gaaggtgggc atgatgcctg
     1081 ggaagcagaa acaagccagg cagatgcacc ccttgccttg cttccgaagg gctgcagtag
     1141 catggaaaac atggaaaaca accaatccat tccctttgct gatataacag gctccaaagc
     1201 caaaacctgt cactggaggc tcaagagcag atctccagcc aagaggcaaa ggaatggggg
     1261 aagctggagg gcctccctct ggttatccag gcttctgaag gttcaagcaa agaaagggtt
     1321 acaaccttaa aaggagagcg tcccggggta tgggtagaag actgctccac cccgaccccc
     1381 agggtcccta accgtctttt ccctgggcga gtcagcccaa tcacaggact gagagtgcct
     1441 ctttagtagc agcaagccac ttcggacacc caaatggaac acctccagtc agccctcgcc
     1501 gaccacccca ccccctccat ccttttccct cagcctccga ttggctgaat ctagagtccc
     1561 tccctgctcc cccctctctc cccacccctg gtaaaactgc gggcttcagg ctgggtgcag
     1621 caactggagg cgttggcgac caggaggagg ctgcagctag gggagtccag gtgagagcag
     1681 gccgacggga gggacccgca catgcaagga ccgccgcagg gcgaggatgc aagccttccc
     1741 cagctacagt tttgggaaag gataccaggg cgctcctata tgggggcgcg ggaacctggg
     1801 gaaagaaggt gctcccaggt cgaggtggga gaggaaggca gtgcggggtc acgggctttc
     1861 tccctgctaa cggacgcttt cgaagagtgg gtgccggaga gaaccatgag gaaggacatc
     1921 aaggacagcc tttggtcccc aagctcaaat cgctttagtg gtgcgaatag agggaggagg
     1981 tgggtggcaa actggaggga gtccccagcg gtgacctcgt ggctggctgg tgcggggcac
     2041 caggtaagaa acgcaatgtt gcgggagggg actgggtggc aggcgcgggg gaggggaaag
     2101 ctagaaagga tgcgagggag cggagggggg agggagcggg ggaatctcaa ctggtagagg
     2161 aaagttaaaa tgaggaaata gcatcagggt ggggttagcc aagccgggcc tcagggaaag
     2221 ggcgcaaagt ttgtctgggt gtgggcttag gtgggctggg tatgagattc ggggcgccga
     2281 aaacactgct gcgcctctgc caaatcacgc tacccctgta tctagttctg ctaggcttct
     2341 ccagccccag ccccaattct tttctcagtg tccccttccc tcccctgaat ctcaagccca
     2401 cactccctcc tccataaccc actgttatca aatctaagtc atttgccacc caacaaccat
     2461 caggaggcgg aagcagacgg gaggagtttg agatcaactt gggctacatc acgagttcca
     2521 agctcaccaa ggcttcttaa ggagaccttg tctctaaaat taattaatta attaattaat
     2581 agtccccttt ctctgccaca gaaccttggg atctggctcc tggtcgcagc tccccccacc
     2641 ccaggctgac attcactgcc atagcccatc cggaaatcct agtctattcc ccatggatct
     2701 tgaactgcag agagaatggc agagtggccc gccctgtgca aaggatgttc ctagcctagg
     2761 tggagctcgc gaactcggag actgtgcctc tcttgggcaa ggacaggcta gacagcctgc
     2821 cggtgttgag ctagggcact gtggggaagg cagagaacct gtgcagggca cgcaatgaac
     2881 acaggaccag aaaactgcag ccctaggaac actcaagagc tggccatttg caagcatctc
     2941 tggcctccgt gcttctcact catgtcccat gtcttataca ggcctctgtg gcacctcgct
     3001 tgcctgatct catccctagc cgttaagctt tctgcatgac ttatcacttg gggcataatg
     3061 ctggatacct accattttct tagaccccat caaaatccta tttgagtgta cggttcggag
     3121 aactccttat ttatccggta aatgtctttt actctgctct cagggagctg aggcaggaca
     3181 ttcctgagat acattgggag aggaatacag tttcaataaa ataataggtt gggtggaggt
     3241 acatgcctat aatgccacca ctcaggaaat ggtggcagct tcgtgagttt gaggccaacc
     3301 caagaaacat agtgaaaccc tgtcagtaaa taagtaagca agtatttgag tatctactat
     3361 atgctagggc tgacctggac attaggggtc atcttctgaa caaactagtg cttgagggag
     3421 gtatttgggg tttttgtttg tttaatggat ctgaatgagt tccagagact ggctacacag
     3481 cgatatgact gagcttaaca cccctaaagc atacagtcag accaattaga caataaaagg
     3541 tatgtatagc ttaccaaata aaaaaattgt attttcaaga gagtgtctgt ctgtgtagcc
     3601 ctggctgttc ttgaactcac tctgtagacc aggctggcct ggaaatccat ctgcctctgc
     3661 ctctctgcct ctctgcctct ctgcctctct ctctgcctct ctctgcctct ctctgcccct
     3721 ctctgcccct ctctgcccct ctctgccgcc ctctgccttt tgccctctgc cctctgttct
     3781 ctggcctctg ccctctccct gccttctccc tctgcctctg cctcttgagt gctggaatca
     3841 aaggtgtgag ctctataggt cttaagttcc agaagaaacg taatgaagtc acccagcagg
     3901 aggtgctcag ggacagcaag acacacacac ccaggacact aggctcccac ttccttggct
     3961 ttctctgagt ggcaaaggac cttaggcagt gtcactccct aagagaaggg gataaagaga
     4021 ggggctgagg tattcatcat gtgctccgtg gatctcaagc cctcaaggta aatggggacc
     4081 cacctgtcct accagctggc tgacctgtag ctttccccac cacagaatcc aagtcggaac
     4141 tcttggcacc tagaggatct cgagccgcca ccatgcctaa gaagaagagg aaggtttacc
     4201 catacgatgt tccagattac gctggatcct ctgagctgat tattagtggt tcatctggtg
     4261 gattcctgcg aaacatcggc aaagagtatc aggaggccgc tgaaaacttc atgaggttta
     4321 tgaatgacca gggggcgtac gctcctaaca ctttgaggga tttgaggttg gtctttcata
     4381 gctgggccag atggtgccat gctcggcagc ttgcatggtt tccaattagt cctgaaatgg
     4441 cacgcgaata ctttcttcag ttgcacgatg cagacctggc ctccactacc atcgacaagc
     4501 actatgctat gcttaatatg cttctgtccc actgcggact gccacccttg tccgacgaca
     4561 agtcagtgag tcttgccatg agaagaatta gaagagaagc cgcaaccgaa aagggtgaga
     4621 ggacaggaca ggcaatcccc ctgcgctggg acgacctgaa gctgctggat gtgctgctca
     4681 gcaggagcga gcggctggtc gacctgcgca acagggcttt cctgttcgta gcctataaca
     4741 ccctcatgag aatgtctgaa atatcacgca tcagggttgg ggacttggat cagacaggag
     4801 acacagtgac cctgcacatc agtcacacta agacaatcac cacagctgcg ggccttgaca
     4861 aagtgctctc ccggcgaacc acagcagtgc tcaatgactg gctggacgtc agtgggctta
     4921 gagaacatcc agacgctgtg ctcttcccac ctatacaccg gtcaaacaaa gcccgcatta
     4981 ctaccacgcc cctgaccgcc cctgccatgg agaagatttt cagtgatgcc tgggtgctgc
     5041 tgaacaaacg ggacgccacc cccaataaag ggaggtatag gacctggacc ggccattccg
     5101 ccagggtggg tgccgcaata gacatggccg agaaacaggt gtctatggtc gagattatgc
     5161 aggaagggac atggaagaag cctgaaacac tgatgcggta tctcagaagg ggcggagtgt
     5221 ccgtgggagc caattctcga ctgatggata gcggcagcgg cgccacgaac ttctctctgt
     5281 taaagcaagc aggcgacgtg gaagaaaacc ccggtcccag cgagctgatt aaggagaaca
     5341 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     5401 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     5461 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     5521 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     5581 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     5641 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     5701 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag accctgtacc
     5761 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     5821 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
     5881 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     5941 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     6001 ggcacaagct gaattaggtc agagctcgag gtccttcctc tgcagaggtc ttgcttctcc
     6061 cggtcagctg actccctccc caagtccttc aaatatctca gaacatgggg agaaacgggg
     6121 accttgtccc tcctaaggaa ccccagtgct gcatgccatc atccccccca ccctcgcccc
     6181 cacccccgcc acttctccct ccatgcatac cactagctgt cattttgtac tctgtattta
     6241 ttctagggct gcttctgatt atttagtttg ttctttccct ggagacctgt tagaacataa
     6301 gggcgtatgg tgggtagggg aggcaggata tcagtcccct ggggcgagtt cctccctgcc
     6361 aaccaagcca gatgcctgaa agagatatgg atgagggaag ttggactgtg cctgtacctg
     6421 gtacagtcat actctgttga aagaatcatc ggggaggggg gggggctcaa gaggggagag
     6481 ctctctgagc ctttgtggac catccaatga ggatgagggc ttagattcta ccaggtcatt
     6541 ctcagccacc acacacaagc gctctgccat cactgaagaa gccccctagg gctcttgggc
     6601 cagggcacac tcagtaaaga tgcaggttca gtcagggaat gatggggaaa ggggtaggag
     6661 gtgggggagg gatcaccccc tcctctaaaa cacgagcctg ctgtctccaa aggcctctgc
     6721 ctgtagtgag ggtggcagaa gaagacaagg agccagaact ctgactccag gatctaagtc
     6781 cgtgcaggaa ggggatccta gaaccatctg gttggaccca gcttaccaag ggagagcctt
     6841 tattccttct ttcccttgcc cctctgtgcc agcccctctt gctgtccctg atcccccaga
     6901 cagcgagagt cttgcaacct gcctcttcca agacctccta atctcagggg caggcggtgg
     6961 agtgagatcc ggcgtgcaca ctttttggaa gatagctttc ccaaggatcc tctcccccac
     7021 tggcagctct gcctgtccca tcaccatgta taataccacc actgctacag catctcaccg
     7081 aggaaagaaa actgcacaat aaaaccaagc ctctggagtg tgtcctggtg tctgtctctt
     7141 ctgtgtcctg gcgtctgtct cttctgtgtt cttccaaggt cagaaacaaa aaccacacac
     7201 ttcaacctgg atggctcggc tgagcacttc tgtgtgcaga aggtccaacc agactctggg
     7261 gtaccccggc cctccctatt cccttgcctc ctgtctcccg ctttttatag ctccctatgc
     7321 tgggcttctc tggagagtga aatctttgcc caaatcaatg cgcattctct ctgctgagtc
     7381 atctggcgac agcagttgag ttcacccgcc aacacatggg cccagctatg tagccgaacc
     7441 ctggctctgg aagtgccagg gactttgtgc ataagtatgt accatgccct tttttcacag
     7501 tcctagctct gcagaagtgc agcctgaagg cctgtctgct gagaggacat gccctggagc
     7561 cctgaaacag gcacagtggg aggaggaacg gaggatgaca ggcatcaggc cctcagtcca
     7621 aaagcaacca cttgagaatg ggctggagta cgaaacatgg ggtcccgtcc ctggatccct
     7681 cctcaaagag taataagtaa aatataaaca ggtaccccag gccgttctgg gtttgggttg
     7741 taatgggatc catttgcaga gaactattga gacagcccag ccgtactgtg acaggcaatg
     7801 tgggggagga ggttgaatca cttggtattt agcatgaata gaataattcc ctgaacattt
     7861 ttcttaaaca tccatatcta aattaccacc actcgctccc agtcttcctg cctttgcgcc
     7921 agcctcctgt ctggccatgc ctgaagaagg ctggagaagc cacccacctc aggccatgac
     7981 actgccagcc acttggcagg tgcagccaaa cctgagctgt cccagaaagg gacattctca
     8041 agacccaggc accctgatca gcactgactt ggagctacaa gtgtcatgcc agaaaagtct
     8101 ctaagaaaac cttttcaggg aaaagggggt gactcaacac cgggcaagtt tgggaagccc
     8161 cacccttcga gtgatggaag agcagatagg aagcctcaga agagagacac cggcacccag
     8221 gtaacgttcc tcatgtggtc tctgtcacac taggtgctct tccctggaca tctccgtgac
     8281 cacactctca gttcttaggg agatgcgggt gctctctgag gctatctcag agttgcagat
     8341 tctgaggcct agagtgacta cagtcagcct aggaagccac agaggactgt ggaccaggag
     8401 ggcagaagag gagaagggaa gaaaaaccat cagataggac ttgcaatgaa actaacccaa
     8461 gacaatcata atgcagacag gaatgttaaa ggcgttcagc agctggccat gacacccatc
     8521 tgtccctctg gccaagtcag caagcctgga agacctggga ctcctgccca tatgtcctaa
     8581 gctccccacc cacccactcg ttcactgtcc ttattctctc tctaccttca gccacttagt
     8641 ttcctacctt aagtcctaga attgatcctg gcgtaatagc gaagaggccc gcaccgatcg
     8701 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     8761 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     8821 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     8881 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     8941 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     9001 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     9061 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     9121 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     9181 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     9241 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     9301 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     9361 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     9421 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     9481 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     9541 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     9601 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     9661 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     9721 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     9781 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9841 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9901 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9961 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    10021 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    10081 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    10141 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    10201 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    10261 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    10321 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
    10381 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    10441 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    10501 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    10561 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    10621 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    10681 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    10741 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    10801 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    10861 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    10921 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    10981 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca ggatcgcggc
    11041 cgcgatcccc ggg
//
