LOCUS       sequence-77901-e       11157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the Dre-dependent Roxed-Cre gene under the control of 
            the Thy1.2 expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11157)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 11157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: Thy1.2-Roxed-Cre
FEATURES             Location/Qualifiers
     source          1..11157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4177..4197
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4322..4353
                     /label=rox
                     /bound_moiety="Dre recombinase"
     polyA_signal    4541..4675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4795..4929
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5049..5183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    5212..5243
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             6096..6119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8911..8930)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9030..9052
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9090..9108)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9176..9280
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9281..9349,9350..10141)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9499..9518)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10312..10900
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10801..10820
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11054..11071
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgagctcga attcagagac cgggaaccaa actagccttt aaaaaacata agtacaggag
       61 ccagcaagat ggctcagtgg gtaaaggtgc ctaccagcaa gcctgacagc ctgagttcag
      121 tccccacgaa ctacgtggta ggagaggacc aaccaactct ggaaatctgt tctgcaaaca
      181 catgctcaca cacacacaca caaatagtat aaacaatttt aaatttcatt taaaaataat
      241 ttgtaaacaa aatcattagc acaggtttta gaaagagcct cttggtgaca tcaagttgat
      301 gctgtagatg gggtatcatt cctgaggacc caaaaccggg tctcagcctt tccccattct
      361 gagagttctc tcttttctca gccactagct gaagagtaga gtggctcagc actgggctct
      421 tgagttccca agtcctacaa ctggtcagcc tgactactaa ccagccatga agaaacaagg
      481 agtggatggg ctgagtctgc tgggatggga gtggagttag taagtggcca tggatgtaat
      541 gaccccagca atgctggcta gaaggcatgc ctcctttcct tgtctggaga cggaacggga
      601 tcatcttgta ctcacagaag ggagaacatt ctagctggtt gggccaaaat gtgcaagttc
      661 acctggaggt ggtggtgcat gcttttaact ccagtactca ggaggcaggg ccaggtggat
      721 ctctgtgagt tcaagaccag cctgcactat ggagagagtt ttgggacagc cagagttaca
      781 cagaaaaatc ctggtggaaa atctgaaaga aagagagaaa gaaagaaaga aagaaagaaa
      841 gaaagaaaga aagaaagaaa gaaagaaaga aagaaagaaa ggaagaaaga aagaaagagt
      901 ggcaggcagg caggcaggag gaaggaagga aggaaggaag gaaggaagga aggaaggaag
      961 gaaggaagga aggaaggaag gaaaataggt gcgacttcaa gatccggagt tacaagcaga
     1021 atgcactgtt tccctaacag ggccaagtgt tttgagtaac tgaaggtggg catgatgcct
     1081 gggaagcaga aacaagccag gcagatgcac cccttgcctt gcttccgaag ggctgcagta
     1141 gcatggaaaa catggaaaac aaccaatcca ttccctttgc tgatataaca ggctccaaag
     1201 ccaaaacctg tcactggagg ctcaagagca gatctccagc caagaggcaa aggaatgggg
     1261 gaagctggag ggcctccctc tggttatcca ggcttctgaa ggttcaagca aagaaagggt
     1321 tacaacctta aaaggagagc gtcccggggt atgggtagaa gactgctcca ccccgacccc
     1381 cagggtccct aaccgtcttt tccctgggcg agtcagccca atcacaggac tgagagtgcc
     1441 tctttagtag cagcaagcca cttcggacac ccaaatggaa cacctccagt cagccctcgc
     1501 cgaccacccc accccctcca tccttttccc tcagcctccg attggctgaa tctagagtcc
     1561 ctccctgctc ccccctctct ccccacccct ggtaaaactg cgggcttcag gctgggtgca
     1621 gcaactggag gcgttggcga ccaggaggag gctgcagcta ggggagtcca ggtgagagca
     1681 ggccgacggg agggacccgc acatgcaagg accgccgcag ggcgaggatg caagccttcc
     1741 ccagctacag ttttgggaaa ggataccagg gcgctcctat atgggggcgc gggaacctgg
     1801 ggaaagaagg tgctcccagg tcgaggtggg agaggaaggc agtgcggggt cacgggcttt
     1861 ctccctgcta acggacgctt tcgaagagtg ggtgccggag agaaccatga ggaaggacat
     1921 caaggacagc ctttggtccc caagctcaaa tcgctttagt ggtgcgaata gagggaggag
     1981 gtgggtggca aactggaggg agtccccagc ggtgacctcg tggctggctg gtgcggggca
     2041 ccaggtaaga aacgcaatgt tgcgggaggg gactgggtgg caggcgcggg ggaggggaaa
     2101 gctagaaagg atgcgaggga gcggaggggg gagggagcgg gggaatctca actggtagag
     2161 gaaagttaaa atgaggaaat agcatcaggg tggggttagc caagccgggc ctcagggaaa
     2221 gggcgcaaag tttgtctggg tgtgggctta ggtgggctgg gtatgagatt cggggcgccg
     2281 aaaacactgc tgcgcctctg ccaaatcacg ctacccctgt atctagttct gctaggcttc
     2341 tccagcccca gccccaattc ttttctcagt gtccccttcc ctcccctgaa tctcaagccc
     2401 acactccctc ctccataacc cactgttatc aaatctaagt catttgccac ccaacaacca
     2461 tcaggaggcg gaagcagacg ggaggagttt gagatcaact tgggctacat cacgagttcc
     2521 aagctcacca aggcttctta aggagacctt gtctctaaaa ttaattaatt aattaattaa
     2581 tagtcccctt tctctgccac agaaccttgg gatctggctc ctggtcgcag ctccccccac
     2641 cccaggctga cattcactgc catagcccat ccggaaatcc tagtctattc cccatggatc
     2701 ttgaactgca gagagaatgg cagagtggcc cgccctgtgc aaaggatgtt cctagcctag
     2761 gtggagctcg cgaactcgga gactgtgcct ctcttgggca aggacaggct agacagcctg
     2821 ccggtgttga gctagggcac tgtggggaag gcagagaacc tgtgcagggc acgcaatgaa
     2881 cacaggacca gaaaactgca gccctaggaa cactcaagag ctggccattt gcaagcatct
     2941 ctggcctccg tgcttctcac tcatgtccca tgtcttatac aggcctctgt ggcacctcgc
     3001 ttgcctgatc tcatccctag ccgttaagct ttctgcatga cttatcactt ggggcataat
     3061 gctggatacc taccattttc ttagacccca tcaaaatcct atttgagtgt acggttcgga
     3121 gaactcctta tttatccggt aaatgtcttt tactctgctc tcagggagct gaggcaggac
     3181 attcctgaga tacattggga gaggaataca gtttcaataa aataataggt tgggtggagg
     3241 tacatgccta taatgccacc actcaggaaa tggtggcagc ttcgtgagtt tgaggccaac
     3301 ccaagaaaca tagtgaaacc ctgtcagtaa ataagtaagc aagtatttga gtatctacta
     3361 tatgctaggg ctgacctgga cattaggggt catcttctga acaaactagt gcttgaggga
     3421 ggtatttggg gtttttgttt gtttaatgga tctgaatgag ttccagagac tggctacaca
     3481 gcgatatgac tgagcttaac acccctaaag catacagtca gaccaattag acaataaaag
     3541 gtatgtatag cttaccaaat aaaaaaattg tattttcaag agagtgtctg tctgtgtagc
     3601 cctggctgtt cttgaactca ctctgtagac caggctggcc tggaaatcca tctgcctctg
     3661 cctctctgcc tctctgcctc tctgcctctc tctctgcctc tctctgcctc tctctgcccc
     3721 tctctgcccc tctctgcccc tctctgccgc cctctgcctt ttgccctctg ccctctgttc
     3781 tctggcctct gccctctccc tgccttctcc ctctgcctct gcctcttgag tgctggaatc
     3841 aaaggtgtga gctctatagg tcttaagttc cagaagaaac gtaatgaagt cacccagcag
     3901 gaggtgctca gggacagcaa gacacacaca cccaggacac taggctccca cttccttggc
     3961 tttctctgag tggcaaagga ccttaggcag tgtcactccc taagagaagg ggataaagag
     4021 aggggctgag gtattcatca tgtgctccgt ggatctcaag ccctcaaggt aaatggggac
     4081 ccacctgtcc taccagctgg ctgacctgta gctttcccca ccacagaatc caagtcggaa
     4141 ctcttggcac ctagaggatc tcgacccgcc accatgccca agaagaagag gaaggtgacc
     4201 tctgatgaag tcaggaagaa cctgatggac atgttcaggg acaggcaggc cttctctgaa
     4261 cacacctgga agatgctcct gtctgtgtgc agatcctggg ctgcctggtg caagctgaac
     4321 gtaactttaa ataattggca ttatttaaag ttaatgtatg cgctacgaag ttattcgcga
     4381 tgaataaatg aaagcttgca gatctgcgac tctagaggat ctgcgactct agaggatcat
     4441 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4501 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4561 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4621 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgcga
     4681 ctctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     4741 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     4801 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4861 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4921 gtctggatct gcgactctag aggatcataa tcagccatac cacatttgta gaggttttac
     4981 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5041 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5101 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5161 atgtatctta tcatgtctgg atccccatca agctgatccg gaacccttaa ttaactttaa
     5221 ataattggca ttatttaaag ttaaacagga aatggttccc tgctgaacct gaggatgtga
     5281 gggactacct cctgtacctg caagccagag gcctggctgt gaagaccatc caacagcacc
     5341 tgggccagct caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg
     5401 ctgtgtccct ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca
     5461 agcaggccct ggcctttgaa cgcactgact ttgaccaagt cagatccctg atggagaact
     5521 ctgacagatg ccaggacatc aggaacctgg ccttcctggg cattgcctac aacaccctgc
     5581 tgcgcattgc cgaaattgcc agaatcagag tgaaggacat ctcccgcacc gatggtggga
     5641 gaatgctgat ccacattggc aggaccaaga ccctggtgtc cacagctggt gtggagaagg
     5701 ccctgtccct gggggttacc aagctggtgg agagatggat ctctgtgtct ggtgtggctg
     5761 atgaccccaa caactacctg ttctgccggg tcagaaagaa tggtgtggct gccccttctg
     5821 ccacctccca actgtccacc cgggccctgg aagggatctt tgaggccacc caccgcctga
     5881 tctatggtgc caaggatgac tctgggcaga gatacctggc ctggtctggc cactctgcca
     5941 gagtgggtgc tgccagggac atggccaggg ctggtgtgtc catccctgaa atcatgcagg
     6001 ctggtggctg gaccaatgtg aacattgtga tgaactacat cagaaacctg gactctgaga
     6061 ctggggccat ggtgaggctg ctcgaggatg gggacgatta caaggatgac gacgataagt
     6121 aggtcagagg tcgaggtcct tcctctgcag aggtcttgct tctcccggtc agctgactcc
     6181 ctccccaagt ccttcaaata tctcagaaca tggggagaaa cggggacctt gtccctccta
     6241 aggaacccca gtgctgcatg ccatcatccc ccccaccctc gcccccaccc ccgccacttc
     6301 tccctccatg cataccacta gctgtcattt tgtactctgt atttattcta gggctgcttc
     6361 tgattattta gtttgttctt tccctggaga cctgttagaa cataagggcg tatggtgggt
     6421 aggggaggca ggatatcagt cccctggggc gagttcctcc ctgccaacca agccagatgc
     6481 ctgaaagaga tatggatgag ggaagttgga ctgtgcctgt acctggtaca gtcatactct
     6541 gttgaaagaa tcatcgggga gggggggggg ctcaagaggg gagagctctc tgagcctttg
     6601 tggaccatcc aatgaggatg agggcttaga ttctaccagg tcattctcag ccaccacaca
     6661 caagcgctct gccatcactg aagaagcccc ctagggctct tgggccaggg cacactcagt
     6721 aaagatgcag gttcagtcag ggaatgatgg ggaaaggggt aggaggtggg ggagggatca
     6781 ccccctcctc taaaacacga gcctgctgtc tccaaaggcc tctgcctgta gtgagggtgg
     6841 cagaagaaga caaggagcca gaactctgac tccaggatct aagtccgtgc aggaagggga
     6901 tcctagaacc atctggttgg acccagctta ccaagggaga gcctttattc cttctttccc
     6961 ttgcccctct gtgccagccc ctcttgctgt ccctgatccc ccagacagcg agagtcttgc
     7021 aacctgcctc ttccaagacc tcctaatctc aggggcaggc ggtggagtga gatccggcgt
     7081 gcacactttt tggaagatag ctttcccaag gatcctctcc cccactggca gctctgcctg
     7141 tcccatcacc atgtataata ccaccactgc tacagcatct caccgaggaa agaaaactgc
     7201 acaataaaac caagcctctg gagtgtgtcc tggtgtctgt ctcttctgtg tcctggcgtc
     7261 tgtctcttct gtgttcttcc aaggtcagaa acaaaaacca cacacttcaa cctggatggc
     7321 tcggctgagc acttctgtgt gcagaaggtc caaccagact ctggggtacc ccggccctcc
     7381 ctattccctt gcctcctgtc tcccgctttt tatagctccc tatgctgggc ttctctggag
     7441 agtgaaatct ttgcccaaat caatgcgcat tctctctgct gagtcatctg gcgacagcag
     7501 ttgagttcac ccgccaacac atgggcccag ctatgtagcc gaaccctggc tctggaagtg
     7561 ccagggactt tgtgcataag tatgtaccat gccctttttt cacagtccta gctctgcaga
     7621 agtgcagcct gaaggcctgt ctgctgagag gacatgccct ggagccctga aacaggcaca
     7681 gtgggaggag gaacggagga tgacaggcat caggccctca gtccaaaagc aaccacttga
     7741 gaatgggctg gagtacgaaa catggggtcc cgtccctgga tccctcctca aagagtaata
     7801 agtaaaatat aaacaggtac cccaggccgt tctgggtttg ggttgtaatg ggatccattt
     7861 gcagagaact attgagacag cccagccgta ctgtgacagg caatgtgggg gaggaggttg
     7921 aatcacttgg tatttagcat gaatagaata attccctgaa catttttctt aaacatccat
     7981 atctaaatta ccaccactcg ctcccagtct tcctgccttt gcgccagcct cctgtctggc
     8041 catgcctgaa gaaggctgga gaagccaccc acctcaggcc atgacactgc cagccacttg
     8101 gcaggtgcag ccaaacctga gctgtcccag aaagggacat tctcaagacc caggcaccct
     8161 gatcagcact gacttggagc tacaagtgtc atgccagaaa agtctctaag aaaacctttt
     8221 cagggaaaag ggggtgactc aacaccgggc aagtttggga agccccaccc ttcgagtgat
     8281 ggaagagcag ataggaagcc tcagaagaga gacaccggca cccaggtaac gttcctcatg
     8341 tggtctctgt cacactaggt gctcttccct ggacatctcc gtgaccacac tctcagttct
     8401 tagggagatg cgggtgctct ctgaggctat ctcagagttg cagattctga ggcctagagt
     8461 gactacagtc agcctaggaa gccacagagg actgtggacc aggagggcag aagaggagaa
     8521 gggaagaaaa accatcagat aggacttgca atgaaactaa cccaagacaa tcataatgca
     8581 gacaggaatg ttaaaggcgt tcagcagctg gccatgacac ccatctgtcc ctctggccaa
     8641 gtcagcaagc ctggaagacc tgggactcct gcccatatgt cctaagctcc ccacccaccc
     8701 actcgttcac tgtccttatt ctctctctac cttcagccac ttagtttcct accttaagtc
     8761 ctagaattga tcctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     8821 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     8881 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     8941 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     9001 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     9061 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     9121 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     9181 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     9241 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     9301 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9361 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     9421 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     9481 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     9541 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     9601 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9661 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9721 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9781 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9841 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9901 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9961 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    10021 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    10081 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    10141 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    10201 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10261 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10321 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10381 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10441 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10501 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10561 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10621 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10681 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10741 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10801 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10861 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10921 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    10981 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    11041 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    11101 gcctctcccc gcgcgttggc cgattcatta atgcaggatc gcggccgcga tccccgg
//
