LOCUS       sequence-77903-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SSR2.0 has two target sites in opposite orientation for generate 
            only one cycle of repairing after I-SceI endonuclease expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Gomez-Cabello D, Jimeno S, Fernandez-Avila MJ, Huertas P
  TITLE     New tools to study DNA double-strand break repair pathway choice.
  JOURNAL   PLoS One. 2013 Oct 14;8(10):e77206. doi: 
            10.1371/journal.pone.0077206.
  PUBMED    24155929
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-14"; volume: "8(10):e77206. doi"; pages: " 
            10.1371/journal.pone.0077206"
COMMENT     Sequence Label: SeeSaw Reporter (SSR2.0)
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      457..466
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(508..529)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccgctagcgc taccggtcgc caccatggtg agcggcctgc tgaaggagag tatgcgcatc
       61 aagatgtaca tggagggcac cgtgaacggc cactacttca agtgcgaggg cgagggcgac
      121 ggcaacccct tcgccggcac ccagagcatg agaatccacg tgaccgaggg cgcccccctg
      181 cccttcgcct tcgacatcct ggccccctgc tgcgagtacg gcagcaggac cttcgtgcac
      241 cacaccgccg agatccccga cttcttcaag cagagcttcc ccgagggctt cacctgggag
      301 agaaccacca cctacgagga cggcggcatc ctgaccgccc accaggacac cagcctggag
      361 ggcaactgcc tgatctacaa ggtgaaagtg cacggcacca acttccccgc cgacggcccc
      421 gtgatgaaga acaagagcgg tctagcgcta ccggtcgcca ccatggtgag caagggcgag
      481 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      541 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      601 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      661 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      721 tccgccatgc ccgaaggcta c
//
