LOCUS       sequence-7791-ex       10892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 10892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: pCEP4CyPet-MAMM
FEATURES             Location/Qualifiers
     source          1..10892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(915..2840)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(3275..3293)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3361..3465
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3466..3534,3535..4326)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3684..3703)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4497..5085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4986..5005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5516..5661
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             5704..6741
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(6739..6758)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6783..6831
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        7485..7864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7865..8068
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8019..8039
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8065..8089
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      8129..8138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8135..8137,8138..8140,8141..8854)
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(8441..8460)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    8859..8993
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8883..8902)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8937..8956
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(9136..9158)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      join(9716..10892,1..613)
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
       61 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
      121 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
      181 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
      241 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
      301 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
      361 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
      421 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
      481 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
      541 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
      601 tgtccttttc ctgctggttg ctcccattct taggtgaatt taaggaggcc aggctaaagc
      661 cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat gttttccaac gcgagaaggt
      721 gttgagcgcg gagctgagtg acgtgacaac atgggtatgc ccaattgccc catgttggga
      781 ggacgaaaat ggtgacaaga cagatggcca gaaatacacc aacagcacgc atgatgtcta
      841 ctggggattt attctttagt gcgggggaat acacggcttt taatacgatt gagggcgtct
      901 cctaacaagt tacatcactc ctgcccttcc tcaccctcat ctccatcacc tccttcatct
      961 ccgtcatctc cgtcatcacc ctccgcggca gccccttcca ccataggtgg aaaccaggga
     1021 ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc tgatattgca ggtaggagcg
     1081 ggctttgtca taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt
     1141 tgtaaaaaga ccatgaaata acagacaatg gactccctta gcgggccagg ttgtgggccg
     1201 ggtccagggg ccattccaaa ggggagacga ctcaatggtg taagacgaca ttgtggaata
     1261 gcaagggcag ttcctcgcct taggttgtaa agggaggtct tactacctcc atatacgaac
     1321 acaccggcga cccaagttcc ttcgtcggta gtcctttcta cgtgactcct agccaggaga
     1381 gctcttaaac cttctgcaat gttctcaaat ttcgggttgg aacctccttg accacgatgc
     1441 tttccaaacc accctccttt tttgcgcctg cctccatcac cctgaccccg gggtccagtg
     1501 cttgggcctt ctcctgggtc atctgcgggg ccctgctcta tcgctcccgg gggcacgtca
     1561 ggctcaccat ctgggccacc ttcttggtgg tattcaaaat aatcggcttc ccctacaggg
     1621 tggaaaaatg gccttctacc tggagggggc ctgcgcggtg gagacccgga tgatgatgac
     1681 tgactactgg gactcctggg cctcttttct ccacgtccac gacctctccc cctggctctt
     1741 tcacgacttc cccccctggc tctttcacgt cctctacccc ggcggcctcc actacctcct
     1801 cgaccccggc ctccactacc tcctcgaccc cggcctccac tgcctcctcg accccggcct
     1861 ccacctcctg ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc
     1921 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct
     1981 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     2041 cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     2101 gctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct
     2161 gcccctcctg cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     2221 gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct
     2281 cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct
     2341 cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc
     2401 cctcctcctg ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
     2461 cctgctcctg cccctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     2521 cctcctgctc ctgcccctcc tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca
     2581 ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt ggggtctccg gacaccatct
     2641 ctatgtcttg gccctgatcc tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct
     2701 cgtcctcttc cccgtcctcg tccatggtta tcaccccctc ttctttgagg tccactgccg
     2761 ccggagcctt ctggtccaga tgtgtctccc ttctctccta ggccatttcc aggtcctgta
     2821 cctggcccct cgtcagacat gattcacact aaaagagatc aatagacatc tttattagac
     2881 gacgctcagt gaatacaggg agtgcagact cctgccccct ccaacagccc ccccaccctc
     2941 atccccttca tggtcgctgt cagacagatc caggtctgaa aattccccat cctccgaacc
     3001 atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac tcccgctgaa catcctcaag
     3061 atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg tccccctttt tgctggacgg
     3121 tagggatggg gattctcggg acccctcctc ttcctcttca aggtcaccag acagagatgc
     3181 tactggggca acggaagaaa agctgggtgc ggcctgtgag gatcagctta tcgatgataa
     3241 gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     3301 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     3361 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3421 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3481 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3541 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3601 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3661 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     3721 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3781 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3841 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3901 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3961 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     4021 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4081 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4141 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4201 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4261 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4321 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4381 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5101 gcctttttac ggttcctggc cttttgctgg ccttgaagct gtccctgatg gtcgtcatct
     5161 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
     5221 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc
     5281 gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt
     5341 tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
     5401 ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc atccccgtgg cccgttgctc
     5461 gcgtttgctg gcggtgtccc cggaagaaat atatttgcat gtctttagtt ctatgatgac
     5521 acaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc agtcggggcg
     5581 gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg
     5641 accctgcagc gacccgctta acagcgtcaa cagcgtgccg cagatcccgg ggggcaatga
     5701 gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     5761 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     5821 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     5881 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     5941 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     6001 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     6061 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     6121 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     6181 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     6241 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     6301 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     6361 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     6421 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     6481 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     6541 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     6601 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     6661 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccggatcgg
     6721 gagatggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     6781 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     6841 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     6901 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     6961 tcgcagccaa cgtcggggcg gcaggccctg ccatagccac tggccccgtg ggttagggac
     7021 ggggtccccc atggggaatg gtttatggtt cgtgggggtt attattttgg gcgttgcgtg
     7081 gggtcaggtc cacgactgga ctgagcagac agacccatgg tttttggatg gcctgggcat
     7141 ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa acacccccga
     7201 cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct agtcgaccaa ttctcatgtt
     7261 tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg cgcagaactg
     7321 gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg
     7381 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa
     7441 tatgtacatt tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac
     7501 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     7561 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     7621 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     7681 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     7741 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     7801 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     7861 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
     7921 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     7981 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt
     8041 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatct ctagaagctg
     8101 ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggag aggaactgtt
     8161 cggcggcatc gtgcccatcc tggtggagct ggagggcgac gtgaacggcc acaagttcag
     8221 cgtgagcggc gagggcgagg gcgacgccac ctacggcaag ctgaccctga agttcatctg
     8281 caccaccggc aagctgcccg tgccctggcc caccctggtg accaccctga cctggggcgt
     8341 gcagtgcttc agccggtacc ccgaccacat gaagcagcac gacttcttca agagcgtgat
     8401 gcccgagggc tacgtgcagg agcggaccat cttcttcaag gacgacggca actacaagac
     8461 ccgggccgag gtgaagttcg agggcgacac cctggtgaac cggatcgagc tgaagggcat
     8521 cgacttcaag gaggacggca acatcctggg ccacaagctg gagtacaact acatcagcca
     8581 caacgtgtac atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaaggcccg
     8641 gcacaacatc accgacggca gcgtgcagct ggccgaccac taccagcaga acacccccat
     8701 cggcgacggc cccgtgatcc tgcccgacaa ccactacctg agcacccaga gcgccctgag
     8761 caaggacccc aacgagaagc gggaccacat ggtgctgctg gagttcgtga ccgccgccgg
     8821 catcacccac ggcatggacg aactgtacaa ataataagga tccagacatg ataagataca
     8881 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     8941 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     9001 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     9061 agtaaaacct ctacaaatgt ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg
     9121 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     9181 agcggatgcc gggagcagac aagcccgtca ggcgtcagcg ggtgttggcg ggtgtcgggg
     9241 cgcagccatg aggtcgactc tagaggatcg atgccccgcc ccggacgaac taaacctgac
     9301 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     9361 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     9421 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     9481 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     9541 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     9601 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     9661 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     9721 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     9781 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     9841 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     9901 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     9961 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
    10021 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
    10081 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
    10141 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
    10201 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
    10261 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
    10321 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
    10381 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
    10441 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
    10501 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
    10561 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
    10621 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
    10681 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
    10741 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
    10801 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
    10861 ctatatctgg gtagcatagg ctatcctaat ct
//
