LOCUS       sequence-77911-e        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses an attenuated T7 RNAP under an isopropyl 
            beta-d-1-thiogalactopyranoside (IPTG) inducible control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pN565
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aatagctggc tgaatggtat cttccagcac ttgttgacgg aagccgaact ctttggaccc
       61 gtaagccagc gtcatgactg aactcttagt cacactgcga gtaacaccgt aagccagcca
      121 ttgaccagcc agtgccttag tgcccagctt gactttctca gagatttcac cagtgttctc
      181 atcggtcacg gtaactactt cgttatcggt cccattgatt gcgtctgctt gtagaatctc
      241 gttgactttc ttagcaacaa tcccgtagat gtcctgaacg gtttcactag gaagcaagtt
      301 aaccgcgcga ccacctacct catctcggag catcgcggag aagtgctgga tgccagagca
      361 agacccgtca aacgccagcg gaagggagca gttatagctc aggccgtggt gctgtacccc
      421 agcgtactca aagcagaacg caaggaagca gaacggagaa tcttgctcag cccaccaagt
      481 gttctccagt ggagacttag cgcaagccat gatgttctcg tggttttcct caatgaactt
      541 gatgcgctca gggaacggaa ccttgtcgac acccgcacag tttgcaccgt ggattttcag
      601 ccagtagtaa ccttccttac cgattggttt acctttcgcc agcgtaagca gtcctttggt
      661 catatcgtta ccttgcgggt tgaacattga cacagcgtaa acacgaccgc gccagtccat
      721 gttgtaaggg aaccagatgg ccttatggtt agcaaactta ttggcttgct caagcatgaa
      781 ctcaaggctg atacggcgag acttgcgagc cttgtccttg cggtacacag cagcggcagc
      841 acgtttccac gcggtgagag cctcaggatt catgtcgatg tcttccggtt tcatcgggag
      901 ttcttcacgc tcaatcgcag ggatgtcctc gaccggacaa tgcttccact tggtgattac
      961 gttggcgacc gctaggactt tcttgttgat tttccatgcg gtgttttgcg caatgttaat
     1021 cgctttgtac acctcaggca tgtaaacgtc ttcgtagcgc atcagtgctt tcttactg
//
