LOCUS       sequence-77912-e        9066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector that expresses H2B-cerFP from internal CAG 
            enhancer-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9066)
  TITLE     Lentivirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti.CAG.H2B-cerFP.W.SINloxP
FEATURES             Location/Qualifiers
     source          1..9066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2034..2337
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2340..2615
                     /label=chicken beta-actin promoter
     intron          2616..3625
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3549..3571
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3633..3652
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3743..4120
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4133..4142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4139..4141,4142..4144,4145..4858)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4184..4205)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4445..4464)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4792..4813
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4867..5455
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4920..4940)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5338..5349)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5597..5630)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5648..5828
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5900..6021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5937..5956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5991..6010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6040..6175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6207..6226)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6208..6226)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6236..6253)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6236..6252)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6245..6267)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6397..6852
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6484..6503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6694..6715
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6881..6985
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6986..7054,7055..7846)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7204..7223)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8017..8605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8506..8525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8759..8776
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8893..8914
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8929..8934,8935..8952,8953..8959)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8967..8983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8972..8994
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8991..9007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8991..9007
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9026..9046
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9028..9046
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1921 ttttatcgat cacgagacta gcctcgagaa gcttgataat tctgcaggac ttctagttat
     1981 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2041 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2101 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2161 gactatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2221 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2281 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgggt
     2341 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     2401 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc
     2461 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
     2521 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     2581 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgttg ccttcgcccc
     2641 gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     2701 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     2761 gacggctcgt ttcttttctg tggctgcgtg aaagccttaa agggctccgg gagggccctt
     2821 tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
     2881 tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     2941 tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg ggggggctgc
     3001 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
     3061 gcggcggtcg ggctgtaacc cccccctgca cccccctccc cgagttgctg agcacggccc
     3121 ggcttcgggt gcggggctcc gtagcggggc gtggcgcggg gctcgccgtg ccgggcgggg
     3181 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     3241 gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     3301 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctggcg
     3361 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg aagcggtgcg
     3421 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgg cgccgtcccc
     3481 ttctccatct ccagcctcgg ggctgccgca gggggacggc tgccttcggg ggggacgggg
     3541 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     3601 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     3661 tcattttggc aaagaattga tttgataccg cgggcccggg atccctcgag gaattatcga
     3721 attcgagctc ggtaccgcca ccatgccaga gccagcgaag tctgctcccg ccccgaaaaa
     3781 gggctccaag aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc gcaagcgcag
     3841 ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc accctgacac
     3901 cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca ttttcgagcg
     3961 catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca tcacctccag
     4021 ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc acgccgtgtc
     4081 cgagggtact aaggccatca ccaagtacac cagcgctaag gatccaccgg tcgccaccat
     4141 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     4201 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     4261 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     4321 cgtgaccacc ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     4381 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     4441 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     4501 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     4561 gctggagtac aacgccatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg
     4621 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     4681 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     4741 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     4801 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata
     4861 ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4921 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4981 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5041 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5101 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5161 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5221 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     5281 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5341 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5401 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     5461 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     5521 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatggga
     5581 tcaattcacc atgggaataa cttcgtatag catacattat acgaagttat gcttttttgc
     5641 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5701 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5761 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5821 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     5881 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     5941 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6001 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     6061 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6181 ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     6241 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     6301 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     6361 cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc ggcgcattaa
     6421 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6481 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6541 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6601 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6661 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6721 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6781 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6841 cgtttacaat ttcccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6901 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6961 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7021 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7081 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7141 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7201 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7261 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7321 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7381 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7441 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7501 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7561 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7621 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7681 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7741 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7801 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7861 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7921 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7981 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8041 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8101 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8161 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8221 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8281 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8341 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8401 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8461 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8521 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8581 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8641 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8701 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8761 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     8821 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     8881 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     8941 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9001 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     9061 gctgca
//
