LOCUS       sequence-77914-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cumate-inducible expression of SYFP2-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQY
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     287..308
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    375..402
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    445..472
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
ORIGIN
        1 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
       61 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
      121 tggtcggggt agcgggcgaa gcactgcacg ccgtagccca gggtggtcac gagggtgggc
      181 cagggcacgg gcagcttgcc ggtggtgcag atcagcttca gggtcagctt gccgtaggtg
      241 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      301 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catgcggaag
      361 cttcctctac tagttacaaa cagaccagat tgtctgtttg ttaaccggcg cagttagcgg
      421 cttcgcggcg atccgtcaac catgtacaaa cagaccagat tgtctgtttg ttccggtaca
      481 ttgacatatg tatccgctca tgatacaata accctgatgc tagcagggag agaccccgaa
      541 tggtcatcat gtcgccgaag cgccgcaccc aggccgagcg cgcgatggaa acccagggca
      601 agctgatcgc cgccgccctg ggcgtcctgc gcgagaaggg ctatgccggc ttccgcatcg
      661 ccgacgtccc gggcgccgcc ggcgtcagcc gcggcgccca gtcgcaccac ttcccgacca
      721 agctggaact gctgctggcc accttcgagt ggctgtacga gcagatcacc gagcgcagcc
      781 gcgcccgcct ggccaagctg aagcccgagg acgacgtcat ccagcagatg ctggacgacg
      841 ccgccgagtt cttcctggac gacgacttct cgatctcgct ggacctgatc gtggccgccg
      901 accgcgaccc ggcgctgcgc gagggcatcc agcgcaccgt cgagcgcaac cgcttcgtcg
      961 tcgaggatat gtggctgggc gtgctggtgt cgcgcggcct g
//
