LOCUS       sequence-77916-e        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cumate-inducible expression of SYFP2-tagged fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Kaczmarczyk A, Vorholt JA, Francez-Charlot A
  TITLE     Cumate-inducible gene expression system for sphingomonads and other 
            Alphaproteobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Nov;79(21):6795-802. doi: 
            10.1128/AEM.02296-13. Epub 2013 Aug 30.
  PUBMED    23995928
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.02296-13"; journalName: "Appl Environ Microbiol"; date:
            "2013-11"; volume: "79"; issue: "21"; pages: "6795-802"
COMMENT     Sequence Label: pQY
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..30
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    73..100
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             177..788
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     primer_bind     complement(930..949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttacaaaca gaccagattg tctgtttgtt aaccggcgca gttagcggct tcgcggcgat
       61 ccgtcaacca tgtacaaaca gaccagattg tctgtttgtt ccggtacatt gacatatgta
      121 tccgctcatg atacaataac cctgatgcta gcagggagag accccgaatg gtcatcatgt
      181 cgccgaagcg ccgcacccag gccgagcgcg cgatggaaac ccagggcaag ctgatcgccg
      241 ccgccctggg cgtcctgcgc gagaagggct atgccggctt ccgcatcgcc gacgtcccgg
      301 gcgccgccgg cgtcagccgc ggcgcccagt cgcaccactt cccgaccaag ctggaactgc
      361 tgctggccac cttcgagtgg ctgtacgagc agatcaccga gcgcagccgc gcccgcctgg
      421 ccaagctgaa gcccgaggac gacgtcatcc agcagatgct ggacgacgcc gccgagttct
      481 tcctggacga cgacttctcg atctcgctgg acctgatcgt ggccgccgac cgcgacccgg
      541 cgctgcgcga gggcatccag cgcaccgtcg agcgcaaccg cttcgtcgtc gaggatatgt
      601 ggctgggcgt gctggtgtcg cgcggcctga gccgcgacga cgcggaggac atcctgtggc
      661 tgatcttcaa ctcggtccgc ggcctggcgg tccgctcgct gtggcagaag gacaaggagc
      721 gcttcgagcg cgtccgcaac tcgaccctgg aaatcgcccg cgagcgctac gccaagttca
      781 agcgctgaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1021 ttcgggaagc gtggcgcttt ctcata
//
