LOCUS       sequence-77919-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Hong H, Takahashi K, Ichisaka T, Aoi T, Kanagawa O, Nakagawa M, 
            Okita K, Yamanaka S
  TITLE     Suppression of induced pluripotent stem cell generation by the 
            p53-p21 pathway.
  JOURNAL   Nature. 2009 Aug 27. 460(7259):1132-5.
  PUBMED    19668191
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Aug 27. 460(7259):1132-5."
COMMENT     Sequence Label: pMXs-DsRed Express
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             87..764
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(217..235)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(267..287)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     485..504
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     679..702
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 aaggctgccg accccggggg tggaccatcc tctagactgc cggatctagc tagttaatta
       61 aggatccccg ggtaccggtc gccaccatgg cctcctccga ggacgtcatc aaggagttca
      121 tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      181 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
      241 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt
      301 acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca
      361 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      421 ccctgcagga cggctccttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg
      481 acggccccgt aatgcagaag aagactatgg gctgggaggc ctccaccgag cgcctgtacc
      541 cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct gaagctgaag gacggcggcc
      601 actacctggt ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct
      661 actactacgt ggactccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
      721 agcagtacga gcgcgccgag ggccgccacc acctgttcct gtagcggccg ccagcacagt
      781 ggtcgacgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
      841 ccacctgtag gtttggcaag ctagcttaag taacgccatt tt
//
