LOCUS       sequence-77927-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human connexin 43 with the start methionine codon mutated to
            isoleucine and with wobble mutations conferring resistance against 
            an siRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Smyth JW, Shaw RM
  TITLE     Autoregulation of Connexin43 Gap Junction Formation by Internally 
            Translated Isoforms.
  JOURNAL   Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009.
  PUBMED    24210816
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009."
COMMENT     Sequence Label: pcDNA3.2-Cx43-NoStart
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..55)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    81..105
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actaaccggt acgcgtagaa tcgagaccga ggagagggtt agggataggc ttaccttcga
       61 accgcgggcc ctctagatca accactttgt acaagaaagc tgggtactag atctccaggt
      121 catcaggccg aggtctgctg ctggcacgac tgctggctct gcttgaaggt cgctggtcca
      181 caatggctag tggctgtaat tcatgtccag cagctagttt ttttgaattc tggttatcat
      241 cggggaaatc aaaaggctgt gcatgggagt tagagatggt gcttcccgcc tgccccattc
      301 gattttgttc tgcactgtaa ttagcccagt tttgctcact tgcttgcttg ttgtaattgc
      361 ggcaagaaga attgtttctg tcgccagtaa ccagcttgta cccaggagga gacataggcg
      421 agaggggagc ggttggtgag gagcagccat tgaaataagc atatttttga gacccacagt
      481 ctttggcagg gctcagcgca ccactggtcg catggtaagg gtcgctcttt cccttaaccc
      541 gatccttaac gcccttgaag aaaacataga agagttcaat gatattcaag gccagggaca
      601 ccaaggacac caccagcatg aagatgatga agatggtttt ctccgtgggg cgagagagga
      661 aacagtccac ctgatgtggg cagggatctc ttttgcaagt gtaaacagca ctcaagctga
      721 atccatagat gtaccactgg atcagcaaga aggccacctc aaagatagac ttgaagagga
      781 tactgatgat gtaggttcgc agcaaccccc ctcgcatttt caccttacca tgctcttcaa
      841 taccgtactt gaacttcttt atctcaatct gcttcaagtg catgtccaca ttgacaccat
      901 cagtttgggc aaccttgagt tcttcctctt tcttgttcag tttctcttcc tttcgcatca
      961 catagaacac atgagccagg
//
