LOCUS       sequence-77934-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor 
            based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond 
            to normal cytosolic Ca2+ levels..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Lindenburg LH, Vinkenborg JL, Oortwijn J, Aper SJ, Merkx M
  TITLE     MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor.
  JOURNAL   PLoS One. 2013 Dec 2;8(12):e82009. doi: 
            10.1371/journal.pone.0082009.
  PUBMED    24312622
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-2"; volume: "8(12):e82009. doi"; pages: " 
            10.1371/journal.pone.0082009"
COMMENT     Sequence Label: pCMVMagFRET-1
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..131
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(426..447)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     775..794
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
       61 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      121 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggca
      181 tctctttcca atatccagtc tgtcacaact tcattaaaat cttcaaaggt gattttccct
      241 gtggcttctc tgtcataatc tttaagaatc ttcagtacat cagctttttt tacatcaaac
      301 cccaaggctc tcattgccac cttcagttca tgataatcta ttgcttcatc tttgtctgta
      361 tcaaacagtt caaaagcatc tttaatttct tgtttctgtt cctcgctagt gatcccggcg
      421 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      481 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      541 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
      601 ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtcgctg
      661 atggcgttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      721 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      781 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
      841 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc
      901 ca
//
