LOCUS       sequence-77937-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor 
            based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond 
            to normal Ca2+ levels..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Lindenburg LH, Vinkenborg JL, Oortwijn J, Aper SJ, Merkx M
  TITLE     MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor.
  JOURNAL   PLoS One. 2013 Dec 2;8(12):e82009. doi: 
            10.1371/journal.pone.0082009.
  PUBMED    24312622
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-2"; volume: "8(12):e82009. doi"; pages: " 
            10.1371/journal.pone.0082009"
COMMENT     Sequence Label: pCMVMagFRET-1-NLS
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..152)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(156..176)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(180..200)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(210..920,921..923,924..926))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(252..273)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     601..620
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     860..881
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
       61 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctaga gtcgcggccg
      121 ctttatagat ctacctttct cttctttttt ggatcaactt tacgcttttt ctttggatcc
      181 accttgcgtt ttttcttggg agatccgccc ttgtacagct cgtccatgcc gagagtgatc
      241 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      301 ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      361 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
      421 cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
      481 tggctgttgt agttgtactc aagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      541 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      601 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
      661 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcacatc
      721 aggccgtagc cgaaggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
      781 cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
      841 ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg
      901 aacagctcct cgcccttgct caccatggca tctctttcca atatccagtc tgtcacaact
      961 tcattaaaat cttcaaaggt gattttccct gtggcttctc tgtcataatc tttaagaatc
     1021 ttc
//
