LOCUS       sequence-7794-ex         412 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Li S, Wang L, Berman MA, Zhang Y, Dorf ME
  TITLE     RNAi screen in mouse astrocytes identifies phosphatases that 
            regulate NF-kappaB signaling.
  JOURNAL   Mol Cell. 2006 Nov 17. 24(4):497-509.
  PUBMED    17188031
REFERENCE   2  (bases 1 to 412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Nov 17. 24(4):497-509."
COMMENT     Sequence Label: pBabe-Dual
FEATURES             Location/Qualifiers
     source          1..412
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        30..270
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     30..50
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     201..220
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        complement(316..406)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(376..395)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 ggatgctgga agctatcgat gggcaggaag agggcctatt tcccatgatt ccttcatatt
       61 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
      121 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
      181 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
      241 tcttggcttt atatatcttg tggaaaggac gaaaaaaact gtcttcggat ccgatatcga
      301 agactatttt ttagagtggt ctcatacaga acttataaga ttcccaaatc caaagacatt
      361 tcacgtttat ggtgatttcc cagaacacat agcgacatgc aaatatgaat tc
//
