LOCUS       sequence-77947-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human connexin 43 with M100, M125, and M147 mutated to L and
            wobble mutations conferring resistance against an siRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Smyth JW, Shaw RM
  TITLE     Autoregulation of Connexin43 Gap Junction Formation by Internally 
            Translated Isoforms.
  JOURNAL   Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009.
  PUBMED    24210816
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009."
COMMENT     Sequence Label: pcDNA3.2-Cx43-M100/125/147L-siResist
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..52)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    78..102
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta ccttcgaacc
       61 gcgggccctc tagatcaacc actttgtaca agaaagctgg gtactagatc tccaggtcat
      121 caggccgagg tctgctgctg gcacgactgc tggctctgct tgaaggtcgc tggtccacaa
      181 tggctagtgg ctgtaattca tgtccagcag ctagtttttt tgaattctgg ttatcatcgg
      241 ggaaatcaaa aggctgtgca tgggagttag agatggtgct tcccgcctgc cccattcgat
      301 tttgttctgc actgtaatta gcccagtttt gctcacttgc ttgcttgttg taattgcggc
      361 aagaagaatt gtttctgtcg ccagtaacca gcttgtaccc aggaggagac ataggcgaga
      421 ggggagcggt tggtgaggag cagccattga aataagcata tttttgagac ccacagtctt
      481 tggcagggct cagcgcacca ctggtcgcat ggtaagggtc gctctttccc ttaacccgat
      541 ccttaacgcc cttgaagaaa acatagaaga gttcaatgat attcaaggcc agggacacca
      601 aggacaccac cagcatgaag atgatgaaga tggttttctc cgtggggcga gagaggaaac
      661 agtccacctg atgtgggcag ggatctcttt tgcaagtgta aacagcactc aagctgaatc
      721 catagatgta ccactggatc agcaagaagg ccacctcaaa gatagacttg aagaggatac
      781 tgatgatgta ggttcgcagc aacccccctc gtagtttcac cttaccatgc tcttcaatac
      841 cgtacttgaa cttctttatc tcaatctgct tcaagtgtag gtccacattg acaccatcag
      901 tttgggcaac cttgagttct tcctctttct tgttcagttt ctcttccttt cgtagcacat
      961 agaacacatg agcc
//
