LOCUS       sequence-7795-ex        1285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Tomishige M, Klopfenstein DR, Vale RD
  TITLE     Conversion of Unc104/KIF1A kinesin into a processive motor after 
            dimerization.
  JOURNAL   Science. 2002 Sep 27. 297(5590):2263-7.
  PUBMED    12351789
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2002 Sep 27. 297(5590):2263-7."
COMMENT     Sequence Label: pET_Unc653_GFP_His
FEATURES             Location/Qualifiers
     source          1..1285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..777)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(104..124)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     719..747
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     gap             1237..1246
                     /estimated_length=10
     gap             1265..1274
                     /estimated_length=10
ORIGIN
        1 gcacnctcct tcgggctcgg ttagcagccg gatcngctcg agtcaanggt gatgatggtg
       61 atgtttgtat agttcatcca tgccatgtgt aatcccagca gctgttacaa actcaagaag
      121 gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg tggacaggta
      181 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
      241 tgctagttga acgcttccat cttcaatgtt gtgtctaatt ttgaagttaa ctttgattcc
      301 attcttttgt ttgtctgcca tgatgtatac attgtgtgag ttatagttgt attccaattt
      361 gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac
      421 aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgaa
      481 aaatatagtt ctttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg
      541 tttcatatga tctgggtatc ttgaaaagca ttgaacacca taagtgaaag tagtgacaag
      601 tgttggccat ggaacaggta gttttccagt agtgcaaata aatttaaggg taagttttcc
      661 gtatgttgca tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc
      721 atctaattca acaagaattg ggacaactcc agtgaaaagt tcttctcctt tactcatggt
      781 accagatata taagattgtg ccaaatccac ttgtttttga agattttcaa tcattgattc
      841 atattccctt gtctgatgat acattttttg ttccaattct actttttcnc ntcngtatgg
      901 agatnccatt tctaacattt tcttctccat atctgcnttt anatcaatnc cntgtttgtc
      961 nanaanttcn tgttgagcat attncaatca atgggttgtt cagctatgct gcnantatgc
     1021 ctgcttgcct gcttccgngg atcatanacn gaaaacgtga gtcncnaaaa acncaaatcn
     1081 gnggtaaana nangantgtc nntgtttccn tnaaaacana ncntcgnttc tggcaggcnn
     1141 tnccccncnt naaatccnng aatcnaaacc cnccccaaaa aannnggnaa ntnnctnggg
     1201 gncnncnnnn cccnntnaaa aaaaaanccc nnnnggnnnn nnnnnnaagg gnnnntnnna
     1261 aancnnnnnn nnnnccccna nnnna
//
