LOCUS       sequence-77952-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human connexin 43 with the start methionine codon mutated to
            isoleucine and with wobble mutations conferring resistance against 
            an siRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Smyth JW, Shaw RM
  TITLE     Autoregulation of Connexin43 Gap Junction Formation by Internally 
            Translated Isoforms.
  JOURNAL   Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009.
  PUBMED    24210816
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009."
COMMENT     Sequence Label: pcDNA3.2-Cx43-NoStart
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     16..35
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16..34
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agttaagcta
       61 tcaacaagtt tgtacaaaaa agcaggcttc accataggtg actggagcgc cttaggcaaa
      121 ctccttgaca aggttcaagc ctactcaact gctggaggga aggtgtggct gtcagtactt
      181 ttcattttcc gaatcctgct gctggggaca gcggttgagt cagcctgggg cgacgaacaa
      241 tccgcattcc gctgtaacac tcagcaacct ggttgtgaaa atgtctgcta tgacaagtct
      301 ttcccaatct ctcatgtgcg cttctgggtc ctgcagatca tatttgtgtc tgtacccaca
      361 ctcttgtacc tggctcatgt gttctatgtg atgcgaaagg aagagaaact gaacaagaaa
      421 gaggaagaac tcaaggttgc ccaaactgat ggtgtcaatg tggacatgca cttgaagcag
      481 attgagataa agaagttcaa gtacggtatt gaagagcatg gtaaggtgaa aatgcgaggg
      541 gggttgctgc gaacctacat catcagtatc ctcttcaagt ctatctttga ggtggccttc
      601 ttgctgatcc agtggtacat ctatggattc agcttgagtg ctgtttacac ttgcaaaaga
      661 gatccctgcc cacatcaggt ggactgtttc ctctctcgcc ccacggagaa aaccatcttc
      721 atcatcttca tgctggtggt gtccttggtg tccctggcct tgaatatcat tgaactcttc
      781 tatgttttct tcaagggcgt taaggatcgg gttaagggaa agagcgaccc ttaccatgcg
      841 accagtggtg cgctgagccc tgccaaagac tgtgggtctc aaaaatatgc ttatttcaat
      901 ggctgctcct caccaaccgc tcccctctcg cctatgtctc c
//
