LOCUS       sequence-77953-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human connexin 43 with M100 mutated to L and wobble 
            mutations conferring resistance against an siRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Smyth JW, Shaw RM
  TITLE     Autoregulation of Connexin43 Gap Junction Formation by Internally 
            Translated Isoforms.
  JOURNAL   Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009.
  PUBMED    24210816
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009."
COMMENT     Sequence Label: pcDNA3.2-Cx43-M100L-siResist
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..52
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        33..51
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    81..105
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
       61 gctggctagt taagctatca acaagtttgt acaaaaaagc aggcttcacc atgggtgact
      121 ggagcgcctt aggcaaactc cttgacaagg ttcaagccta ctcaactgct ggagggaagg
      181 tgtggctgtc agtacttttc attttccgaa tcctgctgct ggggacagcg gttgagtcag
      241 cctggggcga cgaacaatcc gcattccgct gtaacactca gcaacctggt tgtgaaaatg
      301 tctgctatga caagtctttc ccaatctctc atgtgcgctt ctgggtcctg cagatcatat
      361 ttgtgtctgt acccacactc ttgtacctgg ctcatgtgtt ctatgtgcta cgaaaggaag
      421 agaaactgaa caagaaagag gaagaactca aggttgccca aactgatggt gtcaatgtgg
      481 acatgcactt gaagcagatt gagataaaga agttcaagta cggtattgaa gagcatggta
      541 aggtgaaaat gcgagggggg ttgctgcgaa cctacatcat cagtatcctc ttcaagtcta
      601 tctttgaggt ggccttcttg ctgatccagt ggtacatcta tggattcagc ttgagtgctg
      661 tttacacttg caaaagagat ccctgcccac atcaggtgga ctgtttcctc tctcgcccca
      721 cggagaaaac catcttcatc atcttcatgc tggtggtgtc cttggtgtcc ctggccttga
      781 atatcattga actcttctat gttttcttca agggcgttaa ggatcgggtt aagggaaaga
      841 gcgaccctta ccatgcgacc agtggtgcgc tgagccctgc caaagactgt gggtctcaaa
      901 aatatgctta tttcaatggc tgctcctcac caaccgctcc cctctcgcct atgtctcc
//
