LOCUS       sequence-77955-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human connexin 43 with M100, M125, and M147 mutated to L and
            wobble mutations conferring resistance against an siRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Smyth JW, Shaw RM
  TITLE     Autoregulation of Connexin43 Gap Junction Formation by Internally 
            Translated Isoforms.
  JOURNAL   Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009.
  PUBMED    24210816
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009."
COMMENT     Sequence Label: pcDNA3.2-Cx43-M100/125/147L-siResist
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
       61 agttaagcta tcaacaagtt tgtacaaaaa agcaggcttc accatgggtg actggagcgc
      121 cttaggcaaa ctccttgaca aggttcaagc ctactcaact gctggaggga aggtgtggct
      181 gtcagtactt ttcattttcc gaatcctgct gctggggaca gcggttgagt cagcctgggg
      241 cgacgaacaa tccgcattcc gctgtaacac tcagcaacct ggttgtgaaa atgtctgcta
      301 tgacaagtct ttcccaatct ctcatgtgcg cttctgggtc ctgcagatca tatttgtgtc
      361 tgtacccaca ctcttgtacc tggctcatgt gttctatgtg ctacgaaagg aagagaaact
      421 gaacaagaaa gaggaagaac tcaaggttgc ccaaactgat ggtgtcaatg tggacctaca
      481 cttgaagcag attgagataa agaagttcaa gtacggtatt gaagagcatg gtaaggtgaa
      541 actacgaggg gggttgctgc gaacctacat catcagtatc ctcttcaagt ctatctttga
      601 ggtggccttc ttgctgatcc agtggtacat ctatggattc agcttgagtg ctgtttacac
      661 ttgcaaaaga gatccctgcc cacatcaggt ggactgtttc ctctctcgcc ccacggagaa
      721 aaccatcttc atcatcttca tgctggtggt gtccttggtg tccctggcct tgaatatcat
      781 tgaactcttc tatgttttct tcaagggcgt taaggatcgg gttaagggaa agagcgaccc
      841 ttaccatgcg accagtggtg cgctgagccc tgccaaagac tgtgggtctc aaaaatatgc
      901 ttatttcaat ggctgctcct caccaaccgc tcccctctcg cctat
//
