LOCUS       sequence-77956-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes C-terminal fragment of human connexin 43 initiating at M213,
            with M281 and M320 mutated to L, and a V5 C-terminal fusion tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Smyth JW, Shaw RM
  TITLE     Autoregulation of Connexin43 Gap Junction Formation by Internally 
            Translated Isoforms.
  JOURNAL   Cell Rep. 2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009.
  PUBMED    24210816
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2013 Nov 5. pii: S2211-1247(13)00578-0. doi: 
            10.1016/j.celrep.2013.10.009."
COMMENT     Sequence Label: pcDNA3.2-GJA1-20K-V5
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(548..572)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             598..639
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(679..698)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    723..772
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 agctatcaca agtttgtaca aaaagcaggc ttcaccatgc tggtggtgtc cttggtgtcc
       61 ctggccttga atatcattga actcttctat gttttcttca agggcgttaa ggatcgggtt
      121 aagggaaaga gcgaccctta ccatgcgacc agtggtgcgc tgagccctgc caaagactgt
      181 gggtctcaaa aatatgctta tttcaatggc tgctcctcac caaccgctcc cctctcgcct
      241 ctatctcctc ctgggtacaa gctggttact ggcgacagaa acaattcttc ttgccgcaat
      301 tacaacaagc aagcaagtga gcaaaactgg gctaattaca gtgcagaaca aaatcgacta
      361 gggcaggcgg gaagcaccat ctctaactcc catgcacagc cttttgattt ccccgatgat
      421 aaccagaatt caaaaaaact agctgctgga catgaattac agccactagc cattgtggac
      481 cagcgacctt caagcagagc cagcagtcgt gccagcagca gacctcggcc tgatgacctg
      541 gagatctacc cagctttctt gtacaaagtg gttgatctag agggcccgcg gttcgaaggt
      601 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagtt
      661 taaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
      721 gacggcaata aaaagacaga ataaaacgca cgggtgttgg ggtcgtttgt tcataaacgc
      781 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
      841 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
      901 ctcgca
//
