LOCUS       sequence-77959-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. Coli N-term HexHis Tagged Protein Expression Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Bower BD, Griffith JD
  TITLE     TRF1 and TRF2 differentially modulate Rad51-mediated telomeric and 
            nontelomeric displacement loop formation in vitro.
  JOURNAL   Biochemistry. 2014 Sep 2;53(34):5485-95. doi: 10.1021/bi5006249. 
            Epub 2014 Aug 21.
  PUBMED    25115914
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi5006249";
            journalName: "Biochemistry"; date: "2014-09-2- 2"; volume: "53"; 
            issue: "34"; pages: "5485-95"
COMMENT     Sequence Label: pTrcHisB-TRF2
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             101..127
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             164..196
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             209..229
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 agcggcactg ctctttaaca atttatcaga caatctgtgt gggcactcga ccggaattat
       61 cgattaactt tattattaaa aattaaagag gtatatatta atgtatcgat taaataagga
      121 ggaataaacc atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg
      181 tggacagcaa atgggtcggg atctgtacga aaacctgtat tttcagggcg atccgagctc
      241 gagatctgcg cgcgatcgat cgggaggagg cgggagtagc gacggcagcg ggcgggcagc
      301 tggcaggcgg gcgtcccgca gtagcgggcg ggcccggcgg gggcgccacg agccggggct
      361 ggggggcccg gcggagcgcg gcgcggggga ggcacggctg gaagaggcag tcaatcgctg
      421 ggtgctcaag ttctacttcc acgaggcgct gcgggccttt cggggtagcc ggtacgggga
      481 cttcagacag atccgggaca tcatgcaggc tttgcttgtc aggcccttgg ggaaggagca
      541 caccgtgtcc cgattgctgc gggttatgca gtgtctgtcg cggattgaag aaggggaaaa
      601 tttagactgt tcctttgata tggaggctga gctcacacca ctggaatcag ctatcaatgt
      661 gctggagatg attaaaacgg aatttacact gacagaagca gtggtcgaat ccagtagaaa
      721 actggtcaag gaagctgctg tcattatttg tatcaaaaac aaagaatttg aaaaggcttc
      781 aaaaattttg aaaaaacata tgtccaagga ccccacaact cagaagctga gaaatgatct
      841 cctgaatatt attcgagaaa agaacttggc ccatcctgtt atccagaact tttcatatga
      901 gacctt
//
