LOCUS       sequence-77966-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning vector for coexpression of gene cloned in between Sbf and 
            Sal sites with BFP in mammalian cells. Made for cloning of 
            meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, 
            Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
  TITLE     megaTALs: a rare-cleaving nuclease architecture for therapeutic 
            genome engineering.
  JOURNAL   Nucleic Acids Res. 2013 Nov 26.
  PUBMED    24285304
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Nov 26."
COMMENT     Sequence Label: pCVL SFFV Sbf-Sal linker
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..745
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MASELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     CDS             749..802
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccgatggcta gcgagctgat
       61 taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa
      121 gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt
      181 ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg
      241 cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc
      301 tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac
      361 ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa
      421 cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga
      481 gacactgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt
      541 gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa
      601 gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc
      661 caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc
      721 tagcaaactg gggcacaagc ttaattcaga gggcagagga agtcttctaa catgcggtga
      781 cgtggaggag aatccgggcc cctgcaggac gtacatcaca cttcacacga gtcgaccgag
      841 catcttaccg ccatttatac ccatatttgt tctgtttttc ttgatttggg tatacattta
      901 aatgttaata gaacaaaatg gtggggcaat catttacatt tttagggata tgtaattact
      961 agttcaggtg tattgccaca agacaaacat gtta
//
