LOCUS       sequence-77968-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for 
            measurement of cotranslational protein degradation, use with 50572.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Turner GC, Varshavsky A
  TITLE     Detecting and measuring cotranslational protein degradation in vivo.
  JOURNAL   Science. 2000 Sep 22;289(5487):2117-20.
  PUBMED    11000112
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2000-09-22"; volume: "289(5487)"; pages: "2117-20"
COMMENT     Sequence Label: pDBD-R
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..18
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2..18
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             38..598
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     CDS             605..631
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttctagaact agtggatccc ccgggctgca ggaattcatg gttcgaccat tgaactgcat
       61 cgtcgccgtg tcccaaaata tggggattgg caagaacgga gacctaccct ggcctccgct
      121 caggaacgag ttcaagtact tccaaagaat gaccacaacc tcttcagtgg aaggtaaaca
      181 gaatctggtg attatgggta ggaaaacctg gttctccatt cctgagaaga atcgaccttt
      241 aaaggacaga attaatatag ttctcagtag agaactcaaa gaaccaccac gaggagctca
      301 ttttcttgcc aaaagtttgg atgatgcctt aagacttatt gaacaaccgg aattggcaag
      361 taaagtagac atggtttgga tagtcggagg cagttctgtt taccaggaag ccatgaatca
      421 accaggccac ctcagactct ttgtgacaag gatcatgcag gaatttgaaa gtgacacgtt
      481 tttcccagaa attgatttgg ggaagtataa acttctccca gaatacccag gcgtcctctc
      541 tgaggtccag gaggaaaaag gcatcaagta taagtttgaa gtctacgaga agaaagacgg
      601 tacctaccca tacgatgttc cagattacgc tgcattttta gcgggtagtg ggtcgaccat
      661 gcagattttc gtcaagactt tgaccggtaa aaccataaca ttggaagttg aatcttccga
      721 taccatcgac aacgttaagt cgaaaattca agacaaggaa ggtatccctc cagatcaaca
      781 aagattgatc tttgccggta ggcagctaga agacggtaga acgctgtctg attacaacat
      841 tcagaa
//
