LOCUS       sequence-77969-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for 
            measurement of cotranslational protein degradation, use with 50571.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Turner GC, Varshavsky A
  TITLE     Detecting and measuring cotranslational protein degradation in vivo.
  JOURNAL   Science. 2000 Sep 22;289(5487):2117-20.
  PUBMED    11000112
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2000-09-22"; volume: "289(5487)"; pages: "2117-20"
COMMENT     Sequence Label: pDBD-M
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..589
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     CDS             596..622
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tagtggatcc cccgggctgc aggaattcat ggttcgacca ttgaactgca tcgtcgccgt
       61 gtcccaaaat atggggattg gcaagaacgg agacctaccc tggcctccgc tcaggaacga
      121 gttcaagtac ttccaaagaa tgaccacaac ctcttcagtg gaaggtaaac agaatctggt
      181 gattatgggt aggaaaacct ggttctccat tcctgagaag aatcgacctt taaaggacag
      241 aattaatata gttctcagta gagaactcaa agaaccacca cgaggagctc attttcttgc
      301 caaaagtttg gatgatgcct taagacttat tgaacaaccg gaattggcaa gtaaagtaga
      361 catggtttgg atagtcggag gcagttctgt ttaccaggaa gccatgaatc aaccaggcca
      421 cctcagactc tttgtgacaa ggatcatgca ggaatttgaa agtgacacgt ttttcccaga
      481 aattgatttg gggaagtata aacttctccc agaataccca ggcgtcctct ctgaggtcca
      541 ggaggaaaaa ggcatcaagt ataagtttga agtctacgag aagaaagacg gtacctaccc
      601 atacgatgtt ccagattacg ctgcattttt agcgggtagt gggtcgacca tgcagatttt
      661 cgtcaagact ttgaccggta aaaccataac attggaagtt gaatcttccg ataccatcga
      721 caacgttaag tcgaaaattc aagacaagga aggtatccct ccagatcaac aaagattgat
      781 ctttgccggt aggcagctag aagacggtag aacgctgtct
//
