LOCUS       sequence-77976-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid compatible with the Golden Gate TALEN cloning system. To 
            clone TAL effectors 3.5 RVDs long. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, 
            Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
  TITLE     megaTALs: a rare-cleaving nuclease architecture for therapeutic 
            genome engineering.
  JOURNAL   Nucleic Acids Res. 2013 Nov 26.
  PUBMED    24285304
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Nov 26."
COMMENT     Sequence Label: pFUSC3
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(90..109)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(91..109)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(119..135)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(351..367)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      431..517
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      609..636
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagga
       61 cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
      121 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      181 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      241 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgccggtc
      301 tcgatagcca ccacttggtc cggggtcagg ttcaggggga gacgcttaag actggccgtc
      361 gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag agtagggaac
      421 tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc gttttatctg
      481 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
      541 tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
      601 aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgg
      661 ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      721 tcgtgccagc tgcattaatg aatcggccaa cgcgcggg
//
