LOCUS       sequence-77984-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid compatible with the Golden Gate TALEN cloning system. To 
            clone TAL effectors 4.5 RVDs long. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, 
            Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
  TITLE     megaTALs: a rare-cleaving nuclease architecture for therapeutic 
            genome engineering.
  JOURNAL   Nucleic Acids Res. 2013 Nov 26.
  PUBMED    24285304
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Nov 26."
COMMENT     Sequence Label: pFUSC4
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(73..92)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(74..92)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(102..118)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(334..350)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      414..500
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      592..619
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(778..795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga ggacctcgag ggggggcccg
       61 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
      121 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      181 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      241 agcctgaatg gcgaatggga cgcgccctgt agcggcgccg gtctcgatag ccaccacttg
      301 gtccggggtc aggttcaggg ggagacgctt aagactggcc gtcgttttac aacgtcgtga
      361 ctgggaaaac atccatgcta gcgttaacgc gagagtaggg aactgccagg catcaaataa
      421 aacgaaaggc tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      481 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg
      541 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca
      601 tcctgacgga tggccttttt gcgtttctac aaactcttcc tggctagcgg tacgcgtatt
      661 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      721 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      781 gctcactgac tcgctgcgct cggtc
//
