LOCUS       sequence-78018-e        5971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  containing 1.2 units of the HBV genome (subtype ayw).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Cha MY, Ryu DK, Jung HS, Chang HE, Ryu WS
  TITLE     Stimulation of hepatitis B virus genome replication by HBx is linked
            to both nuclear and cytoplasmic HBx expression.
  JOURNAL   J Gen Virol. 2009 Apr;90(Pt 4):978-86. doi: 10.1099/vir.0.009928-0. 
            Epub 2009 Mar 4.
  PUBMED    19264639
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gen 
            Virol."; date: "2009-04"; volume: "90(Pt 4):978-86. doi"; pages: " 
            10.1099/vir.0.009928-0. Epub 2009 Mar 4"
COMMENT     Sequence Label: HBV 1.2-mer WT replicon
FEATURES             Location/Qualifiers
     source          1..5971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(640..659)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(641..659)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(678..694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(678..694)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(691..713)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    702..718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(726..732,733..750,751..756))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    771..792
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(909..926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1080..1668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1160..1179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1839..2630,2631..2699))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2462..2481
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2700..2804)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4589..5434
                     /codon_start=1
                     /product="middle (pre-S2/S) envelope protein of hepatitis B
                     virus"
                     /label=HBsAg (pre-S2/S)
                     /note="hepatitis B surface antigen"
                     /translation="MQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVLTTASPLSSI
                     FSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCL
                     GQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCP
                     LIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWAS
                     ARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI
                     "
ORIGIN
        1 catggctgct aggctgtgct gccaactgga tcctgcgcgg gacgtccttt gtttacgtcc
       61 cgtcggcgct gaatcctgcg gacgaccctt ctcggggtcg cttgggactc tctcgtcccc
      121 ttctccgtct gccgttccga ccgaccacgg ggcgcacctc tctttacgcg gactccccgt
      181 ctgtgccttc tcatctgccg gaccgtgtgc acttcgcttc acctctgcac gtcgcatgga
      241 gaccaccgtg aacgcccacc aaatattgcc caaggtctta cataagagga ctcttggact
      301 ctcagcaatg tcaacgaccg accttgaggc atacttcaaa gactgtttgt ttaaagactg
      361 ggaggagttg ggggaggaga ttaggttaaa ggtctttgta ctaggaggct gtaggcataa
      421 attggtctgc gcaccagcac catgcaactt tttcacctct gcctaatcat ctcttgttca
      481 tgtcctactg ttcaagcctc caagctgtgc cttgggtggc tttggggcat ggacatcgac
      541 ccttataaag aatttggagc tactgtggag ttactctcgt ttttgccttc tgacttcttt
      601 ccttcagtac gagatcttct agataccgca agcttgtctc cctatagtga gtcgtattag
      661 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      721 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      781 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      841 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      901 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1081 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1501 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1741 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1801 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     1861 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     1921 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     1981 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2041 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2101 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2161 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2221 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2281 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2341 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2401 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2461 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2521 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2581 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2641 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     2701 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2761 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2821 gtgccacctg acgtcctttg tttacgtccc gtcggcgctg aatcctgcgg acgacccttc
     2881 tcggggtcgc ttgggactct ctcgtcccct tctccgtctg ccgttccgac cgaccacggg
     2941 gcgcacctct ctttacgcgg actccccgtc tgtgccttct catctgccgg accgtgtgca
     3001 cttcgcttca cctctgcacg tcgcatggag accaccgtga acgcccacca aatattgccc
     3061 aaggtcttac ataagaggac tcttggactc tcagcaatgt caacgaccga ccttgaggca
     3121 tacttcaaag actgtttgtt taaagactgg gaggagttgg gggaggagat taggttaaag
     3181 gtctttgtac taggaggctg taggcataaa ttggtctgcg caccagcacc atgcaacttt
     3241 ttcacctctg cctaatcatc tcttgttcat gtcctactgt tcaagcctcc aagctgtgcc
     3301 ttgggtggct ttggggcatg gacatcgacc cttataaaga atttggagct actgtggagt
     3361 tactctcgtt tttgccttct gacttctttc cttcagtacg agatcttcta gataccgcct
     3421 cagctctgta tcgggaagcc ttagagtctc ctgagcattg ttcacctcac catactgcac
     3481 tcaggcaagc aattctttgc tggggggaac taatgactct agctacctgg gtgggtgtta
     3541 atttggaaga tccagcgtct agagacctag tagtcagtta tgtcaacact aatatgggcc
     3601 taaagttcag gcaactcttg tggtttcaca tttcttgtct cacttttgga agagaaacag
     3661 ttatagagta tttggtgtct ttcggagtgt ggattcgcac tcctccagct tatagaccac
     3721 caaatgcccc tatcctatca acacttccgg agactactgt tgttagacga cgaggcaggt
     3781 cccctagaag aagaactccc tcgcctcgca gacgaaggtc tcaatcgccg cgtcgcagaa
     3841 gatctcaatc tcgggaatct caatgttagt attccttgga ctcataaggt ggggaacttt
     3901 actgggcttt attcttctac tgtacctgtc tttaatcctc attggaaaac accatctttt
     3961 cctaatatac atttacacca agacattatc aaaaaatgtg aacagtttgt aggcccactc
     4021 acagttaatg agaaaagaag attgcaattg attatgcctg ccaggtttta tccaaaggtt
     4081 accaaatatt taccattgga taagggtatt aaaccttatt atccagaaca tctagttaat
     4141 cattacttcc aaactagaca ctatttacac actctatgga aggcgggtat attatataag
     4201 agagaaacaa cacatagcgc ctcattttgt gggtcaccat attcttggga acaagatcta
     4261 cagcatgggg cagaatcttt ccaccagcaa tcctctggga ttctttcccg accaccagtt
     4321 ggatccagcc ttcagagcaa acaccgcaaa tccagattgg gacttcaatc ccaacaagga
     4381 cacctggcca gacgccaaca aggtaggagc tggagcattc gggctgggtt tcaccccacc
     4441 gcacggaggc cttttggggt ggagccctca ggctcagggc atactacaaa ctttgccagc
     4501 aaatccgcct cctgcctcca ccaatcgcca gtcaggaagg cagcctaccc cgctgtctcc
     4561 acctttgaga aacactcatc ctcaggccat gcagtggaat tccacaacct tccaccaaac
     4621 tctgcaagat cccagagtga gaggcctgta tttccctgct ggtggctcca gttcaggaac
     4681 agtaaaccct gttctgacta ctgcctctcc cttatcgtca atcttctcga ggattgggga
     4741 ccctgcgctg aacatggaga acatcacatc aggattccta ggaccccttc tcgtgttaca
     4801 ggcggggttt ttcttgttga caagaatcct cacaataccg cagagtctag actcgtggtg
     4861 gacttctctc aattttctag ggggaactac cgtgtgtctt ggccaaaatt cgcagtcccc
     4921 aacctccaat cactcaccaa cctcttgtcc tccaacttgt cctggttatc gctggatgtg
     4981 tctgcggcgt tttatcatct tcctcttcat cctgctgcta tgcctcatct tcttgttggt
     5041 tcttctggac tatcaaggta tgttgcccgt ttgtcctcta attccaggat cctcaacaac
     5101 cagcacggga ccatgccgga cctgcatgac tactgctcaa ggaacctcta tgtatccctc
     5161 ctgttgctgt accaaacctt cggacggaaa ttgcacctgt attcccatcc catcatcctg
     5221 ggctttcgga aaattcctat gggagtgggc ctcagcccgt ttctcctggc tcagtttact
     5281 agtgccattt gttcagtggt tcgtagggct ttcccccact gtttggcttt cagttatatg
     5341 gatgatgtgg tattgggggc caagtctgta cagcatcttg agtccctttt taccgctgtt
     5401 accaattttc ttttgtcttt gggtatacat ttaaacccta acaaaacaaa gagatggggt
     5461 tactctctaa attttatggg ttatgtcatt ggatgttatg ggtccttgcc acaagaacac
     5521 atcatacaaa aaatcaaaga atgttttaga aaacttccta ttaacaggcc tattgattgg
     5581 aaagtatgtc aacgaattgt gggtcttttg ggttttgctg ccccttttac acaatgtggt
     5641 tatcctgcgt tgatgccttt gtatgcatgt attcaatcta agcaggcttt cactttctcg
     5701 ccaacttaca aggcctttct gtgtaaacaa tacctgaacc tttaccccgt tgcccggcaa
     5761 cggccaggtc tgtgccaagt gtttgctgac gcaaccccca ctggctgggg cttggtcatg
     5821 ggccatcagc gcatgcgtgg aaccttttcg gctcctctgc cgatccatac tgcggaactc
     5881 ctagccgctt gttttgctcg cagcaggtct ggagcaaaca ttatcgggac tgataactct
     5941 gttgtcctat cccgcaaata tacatcgttt c
//
