LOCUS       sequence-78024-e        1190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by 
            rice snoRNA U3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Xie K, Yang Y
  TITLE     RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
  JOURNAL   Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 
            Aug 17.
  PUBMED    23956122
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/mp/sst119";
            journalName: "Mol Plant"; date: "2013-11"; volume: "6"; issue: "6"; 
            pages: "1975-83"
COMMENT     Sequence Label: pRGE31
FEATURES             Location/Qualifiers
     source          1..1190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        392..467
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tctttaaaca tacgaacaga tcacttaaag ttcttctgaa gcaacttaaa gttatcaggc
       61 atgcatggat cttggaggaa tcagatgtgc agtcagggac catagcacaa gacaggcgtc
      121 ttctactggt gctaccagca aatgctggaa gccgggaaca ctgggtacgt tggaaaccac
      181 gtgatgtgaa gaagtaagat aaactgtagg agaaaagcat ttcgtagtgg gccatgaagc
      241 ctttcaggac atgtattgca gtatgggccg gcccattacg caattggacg acaacaaaga
      301 ctagtattag taccacctcg gctatccaca tagatcaaag ctgatttaaa agagttgtgc
      361 agatgatccg tggcaggaga ccgaggtctc ggttttagag ctagaaatag caagttaaaa
      421 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgttttag
      481 agctagaaat agcaagttaa aataaggcta gtccgttttt agcgcgtgca tgcctgcagg
      541 tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg ttagagaggc
      601 ttacgcagca gcactcatca agacgatcta cccgagcaat aatctccagg aaatcaaata
      661 ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca tcaagaacac
      721 agagaaagat atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt
      781 gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag ttcccactga
      841 atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac
      901 tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa
      961 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
     1021 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
     1081 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
     1141 atgccatcat tgcgntnaag gaaaggccat cgttgaagat gcctctgccg
//
